Organ-specificity and genotype-dependency of secoiridoid glucosides’ constitutive biosynthesis in Centaurium erythraea Rafn
2018
Authors:
Nikolić, TijanaMatekalo, Dragana
Skorić, Marijana
Novaković, Lazar
Božunović, Jelena
Aničić, Neda
Mišić, Danijela
Contributors
Uzelac, BrankaDocument Type:
Conference object (Published version)
,
© 2018 by the Serbian Plant Physiology Society
Metadata
Show full item recordAbstract:
Plant species Centaurium erythraea Rafn (fam. Gentianaceae) is characterized by the presence
of secoiridoid glucosides (SGs) as dominant secondary metabolites. The SG biosynthetic pathway
has not yet been fully elucidated, despite the great pharmacological importance of this species.
Here, an insight into the SG biosynthesis is achieved by comparing chemical profiles and secoiridoid-
related gene expression patterns of different C. erythraea genotypes and plant organs. The
results revealed that leaves are the main site of secoiridoid biosynthesis and accumulation in C.
erythraea. The key function in the secoiridoid glucoside biosynthetic pathway has been assigned
to genes encoding GES, G8O, 8HGO, 7DLGT and 7DLH2, while for SLS and CPR a potential biosynthetic-
flux regulatory role has been determined. The correlation between the levels of these
genes’ expression and SG content is evident in different plant organs. Also, the analysis of SG
high- and low-productive genotypes of C. erythraea points out that chemical variability existing at
intra-species level is, at least partially, determined by the different patterns of expression of SG-related
genes in different genotypes. Taking into consideration the biological activity of secoiridoid
glucosides, not only is the information obtained in this study of importance for further SG biosynthesis
elucidation, but it also shows a great potential for future biotechnology-based sustainable
production of these valuable metabolites.
Keywords:
Centaurium erythraea Rafn; secoiridoid glycosides; qPCR; UHPLC-MS/MSFunding / projects:
- Physiological, chemical and molecular analysis of the diversity of selected rare and endangered plant species and application of biotechnology for ex situ conservation and production of biologically active compounds (RS-MESTD-Basic Research (BR or ON)-173024)
In:
- Uzelac B, editor. Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia. Belgrade: Serbian Plant Physiology Society; Institute for Biological Research "Siniša Stanković"; Faculty of Biology; 2018. p. 126.