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Xanthones Production in Gentiana dinarica Beck Hairy Root Cultures Grown in Simple Bioreactors
dc.creator | Vinterhalter, Branka | |
dc.creator | Banjac, Nevena | |
dc.creator | Vinterhalter, Dragan | |
dc.creator | Krstić Milošević, Dijana | |
dc.date.accessioned | 2021-09-03T08:04:44Z | |
dc.date.available | 2021-09-03T08:04:44Z | |
dc.date.issued | 2021 | |
dc.identifier.issn | 2223-7747 | |
dc.identifier.uri | https://radar.ibiss.bg.ac.rs/handle/123456789/4309 | |
dc.description.abstract | The hairy root clones of Gentiana dinarica cl-B, cl-D, cl-3, and cl-14 were cultivated in parallel in diverse simple bioreactors, including temporary immersion systems RITA® (TIS RITA®), bubble column bioreactors (BCB), and Erlenmeyer flasks (EF), and evaluated for biomass production and xanthone content. The obtained results showed that TIS RITA® and BCB containing ½ MS medium with 4% sucrose provided equally good growth conditions in which the majority of the clones displayed the higher percentage of dry matter (DM%), and xanthones norswertianin-1-O-primeveroside (nor-1-O-prim) and norswertianin production than those cultivated in EF. Thin and well branched hairy root clone cl-B grown in BCB for 7 weeks was superior regarding all growth parameters tested, including growth index (19.97), dry weight (2.88 g), and DM% (25.70%) compared to all other clones. Cl-B cultured in TIS RITA® contained the highest amount of nor-1-O-prim (56.82 mg per vessel). In BCB with constant aeration, cl-B accumulated the highest norswertianin content reaching 18.08 mg/vessel. The optimized conditions for cultivation of selected G. dinarica hairy root clones in highly aerated TIS RITA® and BCB systems contribute to the development of bioreactor technology designed for the large scale commercial production of xanthones nor-1-O-prim and norswertianin. | sr |
dc.language.iso | en | sr |
dc.publisher | Basel: MDPI | sr |
dc.relation | info:eu-repo/grantAgreement/MESTD/inst-2020/200007/RS// | sr |
dc.rights | openAccess | sr |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.source | Plants | sr |
dc.subject | Transformed roots | sr |
dc.subject | Bubble bioreactor | sr |
dc.subject | TIS RITA® | sr |
dc.subject | Norswertianin | sr |
dc.subject | Sucrose | sr |
dc.title | Xanthones Production in Gentiana dinarica Beck Hairy Root Cultures Grown in Simple Bioreactors | sr |
dc.type | article | sr |
dc.rights.license | BY | sr |
dcterms.abstract | Винтерхалтер, Драган; Винтерхалтер, Бранка; Крстић-Милошевић, Дијана; Бањац, Невена; | |
dc.rights.holder | © 2021 by the authors. Licensee MDPI, Basel, Switzerland. | sr |
dc.citation.issue | 8 | |
dc.citation.volume | 10 | |
dc.identifier.doi | 10.3390/plants10081610 | |
dc.identifier.pmid | 34451654 | |
dc.identifier.scopus | 2-s2.0-85111728855 | |
dc.identifier.wos | 000689951100001 | |
dc.citation.apa | Vinterhalter, B., Banjac, N., Vinterhalter, D., & Krstić-Milošević, D. (2021). Xanthones Production in Gentiana dinarica Beck Hairy Root Cultures Grown in Simple Bioreactors. Plants, 10(8), 1610. | |
dc.citation.vancouver | Vinterhalter B, Banjac N, Vinterhalter D, Krstić-Milošević D. Xanthones Production in Gentiana dinarica Beck Hairy Root Cultures Grown in Simple Bioreactors. Plants. 2021;10(8):1610. | |
dc.citation.spage | 1610 | |
dc.type.version | publishedVersion | sr |
dc.identifier.fulltext | https://radar.ibiss.bg.ac.rs/bitstream/id/8669/bitstream_8669.pdf | |
dc.citation.rank | M21 |