Combination of high sucrose concentration with exogenuous cytokinins affects endogenous phytohormone profiles of kohlrabi seedling explants during de novo organogenesis
2018
Аутори:
Ćosić, TatjanaMotyka, Vaclav
Savić, Jelena
Cingel, Aleksandar
Raspor, Martin
Devrnja, Nina
Dobrev, Petre I.
Ninković, Slavica
Остала ауторства
Uzelac, BrankaТип документа:
Конференцијски прилог (Објављена верзија)
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© 2018 by the Serbian Plant Physiology Society
Метаподаци
Приказ свих података о документуАпстракт:
Fluctuations in local phytohormone levels are considered to be important for initiation of developmental events in the process of plant organogenesis. Simultaneously, sugars as signaling molecules affect plant metabolism and growth, potentially interacting with hormonal regulation. Thus, the aim of presented study was to see whether there were any changes in cytokinin (CK) homeostasis in 4 different stages (T1-T4) during de novo shoot organogenesis of kohlrabi (Brassica oleracea var. gongylodes cv. Vienna Purple) seedlings under the influence of exogenous CKs, trans-zeatin (transZ) and thidiazuron (TDZ), combined with high sucrose concentration. Explants grown on CK-free medium with 3% sucrose were used as control. Generally, analysis of variance showed statistically significant impact of CK treatment and sucrose concentration, as well as their interaction in all investigated stages, including seedling development before calli formation (T1 and T2), calli formation (T3) and when de novo shoots occurred (T4). The obtained CK profiles exposed remarkable increase in total CK levels in samples treated with transZ, particularly when 9% sucrose was used in nutrition medium, reaching up to 50 000 pmol/g FW. This trend was observed for all CK physiological and structural groups. On the other hand, application of a synthetic urea-type CK, TDZ, contributed to little or no increase in the endogenous CK levels regardless the sucrose concentration. Our results demonstrated that phytohormones metabolism may be triggered by sucrose signaling in kohlrabi shoot organogenesis.
Финансирање / пројекти:
- Биотехнологија ин витро - гајене, лековите и угрожене биљне врсте (RS-MESTD-Basic Research (BR or ON)-173015)
- Czech Science Foundation (16-14649S)
У:
- Uzelac B, editor. Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia. Belgrade: Serbian Plant Physiology Society; Institute for Biological Research "Siniša Stanković"; Faculty of Biology; 2018. p. 39-40.