Potassium iodide promotes bud regeneration from the apical root sections of shallot plants
2018
Аутори:
Zdravković-Korać, SnežanaBelić, Maja
Devrnja, Nina
Ćalić, Dušica
Milojević, Jelena
Остала ауторства
Uzelac, BrankaТип документа:
Конференцијски прилог (Објављена верзија)
,
© 2018 by the Serbian Plant Physiology Society
Метаподаци
Приказ свих података о документуАпстракт:
The apical root sections of Allium ascalonicum plants respond to a callus inducing treatment by
forming callus from the root apical meristem. It has been shown in Arabidopsis thaliana that balance between superoxide and hydrogen peroxide (H2O2) in the root tip determines root meristem
size by controlling the transition from cell proliferation to differentiation. Hence, the increased size
of root meristem may affect callus formation and subsequent bud regeneration from the root-tips.
To test this hypothesis, in vitro-grown shallot plants were pretreated with aqueous solutions of
H2O2 (100, 500 or 1000 μM) or potassium iodide (KI, 1 or 10 mM) for 24h. Plants incubated in water
were used as a control. The apical root sections (1 cm) isolated from these plants were cultivated
on callus induction medium, supplemented with 5 μM 2,4-D + 5 μM BA, for 8 weeks and then on
regeneration induction medium, containing 5 μM BA, for 4 weeks. Obtained results indicate the
significant impact of KI on de novo bud regeneration. Explants treated with 1 mM KI regenerated
buds with significantly higher frequency (83.6% vs. 25.2%) and the mean bud number (6.19 vs.
0.21) compared to the control. The frequencies of bud regeneration and the mean bud numbers obtained for H2O2-treated explants were significantly lower compared to KI-treated explants
(43.5% 52.0% and 1.0-2.3, respectively). Assuming that H2O2 decreases size of the root meristem,
as was shown in Arabidopsis, the results obtained in this work suggest that KI, a H2O2 scavenger,
increased bud-forming capacity presumably by increasing the size of root meristem.
Кључне речи:
Allium ascalonicum; Bud regeneration; Hydrogen peroxide; Potassium iodide; ShallotФинансирање / пројекти:
- Биотехнологија ин витро - гајене, лековите и угрожене биљне врсте (RS-MESTD-Basic Research (BR or ON)-173015)
- Grant No. 451- 03-1629/2017/2138
У:
- Uzelac B, editor. Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia. Belgrade: Serbian Plant Physiology Society; Institute for Biological Research "Siniša Stanković"; Faculty of Biology; 2018. p. 39.