Leaf glandular trichomes and secreted material of in vitro-grown tansy (Tanacetum vulgare L.): micromorphology, structure, histochemistry and essential oil analysis
2018
Аутори:
Devrnja, NinaJanošević, Dušica
Tešević, Vele
Savić, Jelena
Zdravković-Korać, Snežana
Milojević, Jelena
Ćalić, Dušica
Остала ауторства
Uzelac, BrankaТип документа:
Конференцијски прилог (Објављена верзија)
,
© 2018 by the Serbian Plant Physiology Society
Метаподаци
Приказ свих података о документуАпстракт:
The morphology and structure of tansy glandular trichomes were characterized for the first
time using scanning electron microscopy and light microscopy, and their secretion was analyzed
by histochemical staining. Leaf indumentum of tansy was covered with non-glandular and glandular trichomes of biseriate type, distributed on both adaxial and abaxial surface. Both types of
trichomes were also distributed along the stem, but with lower density. Light microscopy showed
that biseriate glandular trichomes consisted of two broad basal cells embedded in the epidermis,
a short single-celled stalk, and a large round multicellular head. The histochemical analysis of the
glandular secretion, performed through several staining methods, revealed the complex nature of
the secreted products, including lipids, terpenes, alkaloids and polysaccharides.
GC/MS phytochemical analysis of volatile compounds secreted by glandular trichomes, identified 42 compounds, representing >97% of the total volatiles in EO, obtained by hydrodistillation
in a Clevenger-type apparatus. The monoterpene fraction was dominant, with the prevalence of
oxygenated monoterpenes (38.5%), while the majority of sesquiterpene fraction referred to oxygenated sesquiterpenes (22.6%). Two dominant compounds, which determined the essential oil
chemotype, were trans-thujone (22.7%) and neryl-isovalerate (20.6%).
The essential oil protects the aerial parts of the plant against herbivores and pathogens and the
biological activity of the secreted products indicates their potential as pesticides or pharmaceuticals.
Кључне речи:
Tanacetum vulgare L.; In vitro; Glandular trichomes; Essential oilФинансирање / пројекти:
- Биотехнологија ин витро - гајене, лековите и угрожене биљне врсте (RS-MESTD-Basic Research (BR or ON)-173015)
У:
- Uzelac B, editor. Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia. Belgrade: Serbian Plant Physiology Society; Institute for Biological Research "Siniša Stanković"; Faculty of Biology; 2018. p. 36.