Enhanced gibberellin catabolism promotes somatic embryo induction from spinach apical root sections
2018
Аутори:
Belić, MajaDevrnja, Nina
Ćalić, Dušica
Zdravković-Korać, Snežana
Milojević, Jelena
Остала ауторства
Uzelac, BrankaТип документа:
Конференцијски прилог (Објављена верзија)
,
© 2018 by the Serbian Plant Physiology Society
Метаподаци
Приказ свих података о документуАпстракт:
Gibberellins (GA) are essential for induction of somatic embryogenesis from spinach apical root
sections. To elucidate the role of GA in this process, expression of genes encoding enzymes that
catalyze the final step of GA20 oxidation to bioactive GA, GA3-oxidase (GA3-ox), and GA degradation, GA2-oxidase (GA2-ox), was assessed. In spinach, there is only one GA3-ox and three GA2-ox
(GA2ox 1, GA2 ox2, GA2 ox3). Expression of these genes was tested in the explants cultivated on
noninductive medium (NM), supplemented with 20 μM NAA, inductive medium (IM), containing
20 μM NAA + 5 μM GA3, or plant growth regulator (PGR)-free medium during a 28 d induction
period. Root-tips isolated from seedlings and immediately frozen for RNA isolation were used as a
control. In the explants cultivated on PGR-free medium, expression of GA3-ox increased gradually
up to 10-fold and was constantly higher than in control. GA2-ox1, with an increase of up to 60-fold, was the most highly expressed GA2-ox, while the expression of GA2-ox2 and GA2-ox3 only
slightly increased compared to the control. In the explants cultivated on NM, expression of GA3-ox
decreased slightly until the 7th day of cultivation and then increased up to 2-fold until the end
of the experiment, while the expression of GA2-ox1 and GA2-ox2 was only slightly higher than
in control. However, explants cultivated on IM showed the constant and significant decrease of
GA3-ox (down to 7-fold) and increase of GA2-ox2 (up to 20-fold) expression. Here, we propose that
continuous decrease in GA3-ox and increase in GA2-ox2 expression were favorable conditions for
somatic embryo induction.
Кључне речи:
Gene expression; Gibberellins; Somatic embryogenesis; SpinachФинансирање / пројекти:
- Биотехнологија ин витро - гајене, лековите и угрожене биљне врсте (RS-MESTD-Basic Research (BR or ON)-173015)
- Grant No. 451- 03-1629/2017/2138
У:
- Uzelac B, editor. Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia. Belgrade: Serbian Plant Physiology Society; Institute for Biological Research "Siniša Stanković"; Faculty of Biology; 2018. p. 29-30.