UHPLC-Orbitrap MS characterization of total phenolics in French marigold (Tagetes patula L.) extracts
2018
Аутори:
Gašić, UrošStupar, Sofija
Savić, Jelena
Devrnja, Nina
Ćosić, Tatjana
Ninković, Slavica
Tešić, Živoslav
Остала ауторства
Uzelac, BrankaТип документа:
Конференцијски прилог (Објављена верзија)
,
© 2018 by the Serbian Plant Physiology Society
Метаподаци
Приказ свих података о документуАпстракт:
Aqueous, ethanol and methanol extracts of aerial parts of ornamental French marigold (Tagetes
patula L.), which consist mostly of flavonoids, have been reported to exhibit different biological
activities, including cytotoxic effect on various human cancer cell lines. However, some of the
most potent flavonoids characteristic for the genus Tagetes, such as quercetagetin and patuletin,
are rare in nature. Thus, optimization of extraction process together with employment of sensitive
techniques and detection equipment should be of interest in gaining information about marigold
biologically active compounds. In the present study, separate extracts of leaves and flowers were
obtained from dry plant material by overnight incubation in methanol, dichloromethane or hot
(60 °C) water, followed by ultrasonic bath maceration. UHPLC–LTQ/orbitrap/MS, employed for separation
and identification, revealed the presence of 68 different compounds in total, belonging to
phenolic acids (20 compounds), flavonoid glycosides (37 compounds), and flavonoid aglycones
(11 compounds). The highest number of compounds was detected in methanol extracts, i.e. 55 in
flowers and 60 in leaves, while after the extraction in dichloromethane only 23 compounds were
detected in leaves and 24 in flowers. In both leaves and flowers, the most diversified were flavonoid
glycosides with quercetin and patuletin in 3, 7 or 3,7 di-O- derivativeforms. Aglycon patuletin
was detected in all analyzed extracts, while quercetagetin was detected only in methanol and
dichloromethane extracts of flowers. Obtained data confirmed T. patula extracts as a rich source of
compounds with biological and potential pharmacological values.
Кључне речи:
Tagetes patula L.; UHPLC-Orbitrap MS; Flavonoids; ExtractsФинансирање / пројекти:
- Биотехнологија ин витро - гајене, лековите и угрожене биљне врсте (RS-MESTD-Basic Research (BR or ON)-173015)
- Корелација структуре и особина природних и синтетичких молекула и њихових комплекса са металима (RS-MESTD-Basic Research (BR or ON)-172017)
У:
- Uzelac B, editor. Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia. Belgrade: Serbian Plant Physiology Society; Institute for Biological Research "Siniša Stanković"; Faculty of Biology; 2018. p. 124.