Induction of potato (Solanum tuberosum L.) defense genes by French marigold (Tagetes patula L.) essential oil
2018
Аутори:
Stupar, SofijaTešević, Vele
Stanković, Jovana
Ćosić, Tatjana
Devrnja, Nina
Tubić, Ljiljana
Cingel, Aleksandar
Ninković, Slavica
Vinterhalter, Branka
Savić, Jelena
Остала ауторства
Uzelac, BrankaТип документа:
Конференцијски прилог (Објављена верзија)
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© 2018 by the Serbian Plant Physiology Society
Метаподаци
Приказ свих података о документуАпстракт:
When attacked, plants activate defense mechanisms including volatile emission. Besides having
a pest repellent effect, volatiles can induce defense response in surrounding unharmed plants.
French marigold (Tagetes patula L.) essential oil (EO) was isolated from the above-ground parts
of plants collected from nature by hydrodistillation using Clevenger apparatus. GC/MS analysis
revealed the presence of 42 compounds, of which 97% (32 compounds) have been identified.
The monoterpene fraction was dominant (76.9%) with terpinolene (32.4%) and limonene (14.7%)
as the most abundant compounds. In order to investigate the potential of marigold EO to induce
the defense of potato, we analyzed 3 genes related to the induced resistance (IR): pathogenesis
related PR-2 and PR-5 genes, and proteinase inhibitor Pin2. The potato plants were exposed to EO
volatiles during 4, 8 and 12 h. Two leaves of EO-treated or untreated control plants were collected,
and plants were then additionally injured by mechanically wounding leaves across the leaf surface.
After 24 h of wounding, another two leaves from each plant were collected and expression
of the IR related genes was analyzed by qPCR method. EO induced altered expression of PR genes,
while Pin2 wasn’t affected by the treatment. Mechanical wounding increased the expression of
all genes after 4 and 8 h of EO treatment, with up to 4-fold change in PR-2. However, in plants
exposed to EO for 12 h before wounding, the level of transcription in PR-2 and Pin2 was similar to
that detected in untreated controls. These findings support the efforts for environmentally-safe
pest control in crops by using natural compounds.
Кључне речи:
Solanum tuberosum L.; Tagetes patula L.; Essential oil; Induction of defenseФинансирање / пројекти:
- Биотехнологија ин витро - гајене, лековите и угрожене биљне врсте (RS-MESTD-Basic Research (BR or ON)-173015)
- Биоактивни природни производи самониклих, гајених и јестивих биљака: одређивање структура и активности (RS-MESTD-Basic Research (BR or ON)-172053)
У:
- Uzelac B, editor. Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia. Belgrade: Serbian Plant Physiology Society; Institute for Biological Research "Siniša Stanković"; Faculty of Biology; 2018. p. 58.