Efficient one-step tissue culture protocol for propagation of endemic plant, Lilium martagon var. cattaniae Vis.
2012
Преузимање 🢃
Аутори:
Skorić, MarijanaŽivković, Suzana
Savić, Jelena
Šiler, Branislav
Sabovljević, Aneta
Todorović, Slađana
Grubišić, Dragoljub
Тип документа:
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт:
A rapid, one-step method for direct leafy and rooted bulblets regeneration of Lilium martagon var. cattaniae Vis. was established using seeds as the starting explants for in vitro culture initiation. Adventitious bulblets were regenerated from one scale explants on MS (Murashige and Skoog, 1962) medium supplied with various concentrations of plant growth regulators. The most efficient medium for multiplication was MS supplemented with 0.2 mg/l 6-benzylaminopurine (BAP) and indole-3-acetic acid (IAA) ranging from 0.25 to 2 mg/l. On average, five bulblets were obtained per explant after six weeks in culture. Upon rooting, about 200 plantlets were successfully hardened in the green house with a 95% survival rate. Preliminary experiments indicated that the plantlets from the greenhouse could be successfully used for field cultivation. In vitro propagation of this endemic and protected plant species is of great importance for germplasm conservation.
Кључне речи:
Bulblet; Endemic; In vitro; Lilium martagon var. cattaniae Vis.; ProtocolИзвор:
African Journal of Biotechnology, 2012, 11, 8, 1862-1867Финансирање / пројекти:
- Физиолошка, хемијска и молекуларна анализа диверзитета одабраних ретких и угрожених биљних врста у циљу еx ситу заштите и продукције биолошки активних једињења (RS-MESTD-Basic Research (BR or ON)-173024)