Selection of stable reference genes in Centaurium erythraea Rafn during in vitro somatic embryogenesis and mechanical wounding
2018
Аутори:
Ćuković, KatarinaPaunović, Danijela
Bogdanović, Milica
Dragićević, Milan
Todorović, Slađana
Subotić, Angelina
Simonović, Ana
Остала ауторства
Uzelac, BrankaТип документа:
Конференцијски прилог (Објављена верзија)
,
© 2018 by the Serbian Plant Physiology Society
Метаподаци
Приказ свих података о документуАпстракт:
Centaurium erythraea Rafn (Gentianaceae) is a medicinal plant, rich in secondary metabolites,
mainly secoiridoid glucosides and xanthones, known for numerous beneficial effects on human
health. In addition, C. erythraea possesses remarkable developmental plasticity and is easily cultured
in vitro, so it represents a suitable model system for studying developmental biology. One
of the most notable illustrations of plant cell totipotency is the capability of certain somatic plant
cells to initiate embryogenic development through somatic embryogenesis (SE). It has been proposed
that mechanical wounding during in vitro manipulations of plant material can promote cell
differentiation and somatic embryo development. Our recent research is based on elucidating
the gene expression profiles of centaury tissues in response to wounding and in different SE stages
using quantitative real-time PCR. Considering the fact that these processes exhibit a dynamic
genetic activity, the selection of stable reference genes is paramount in order to obtain unbiased
conclusions. Hereby we report selection of stable reference genes in C. erythraea for studying gene
expression during somatic embryogenesis and wounding. Thirteen frequently used reference
genes were selected and their expression stability was assessed in different developmental stages,
including globular and cotyledonary stages of embryos, as well as wounded tissues. Specific sets
of primers were designed relying on previously obtained next-generation RNA sequencing data.
The results were interpreted using two algorithmic approaches - geNorm and NormFinder - and
the most stable reference genes from a set of tested candidate genes were determined.
Кључне речи:
reference genes; gene expression; somatic embryogenesis; woundingФинансирање / пројекти:
- Физиолошка, хемијска и молекуларна анализа диверзитета одабраних ретких и угрожених биљних врста у циљу еx ситу заштите и продукције биолошки активних једињења (RS-MESTD-Basic Research (BR or ON)-173024)
- Развој и примена биотехнолошких поступака у добијању здравог садног материјала украсних биљака (RS-MESTD-Technological Development (TD or TR)-31019)
- Модификације антиоксидативног метаболизма биљака са циљем повећања толеранције на абиотски стрес и идентификација нових биомаркера са применом у ремедијацији и мониторингу деградираних станишта (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-43010)
У:
- Uzelac B, editor. Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia. Belgrade: Serbian Plant Physiology Society; Institute for Biological Research "Siniša Stanković"; Faculty of Biology; 2018. p. 28.