Response of antioxidative enzymes to drought and salicylic acid application in Impatiens walleriana grown ex vitro
2018
Authors:
Antonić, DraganaMilošević, Snežana
Pantelić, Danijel
Simonović, Ana
Trajković, Milena
Momčilović, Ivana
Subotić, Angelina
Contributors
Uzelac, BrankaDocument Type:
Conference object (Published version)
,
© 2018 by the Serbian Plant Physiology Society
Metadata
Show full item recordAbstract:
Impatiens walleriana is one of the most popular Impatiens species characterized by fleshy, succulent
leaves and a variety of flower colours. The major problem in production and sale of this
ornamental species is related to its tendency to quickly wilt when drought stressed. Since one of
the most devastating consequences of drought is the onset of oxidative stress, we have studied
the responses of antioxidative enzymes to drought and applied salicylic acid (SA) as a potential
stress-alleviating regulator. The I. walleriana seeds were germinated in a growth chamber under
controlled conditions. Plants (8 weeks old) were divided into four groups: (1) regularly watered
plants (W), (2) regularly watered plants with single application of 2 mM SA (WS), (3) plants exposed
to drought (D), and (4) drought-exposed plants with single application of 2 mM SA (DS). The activities
of antioxidative enzymes including superoxide dismutase (SOD), catalase (CAT) and guaiacol
peroxidase (POX) were assessed in all four groups. Our results show that drought and SA (WS, D,
and DS) increase activity of Cu/ZnSOD and MnSOD in comparison to control (W). Total CAT activity
was increased in D, but not in WS and DS groups; however additional CAT isoforms were observed
in these groups. POX activity was generally high in D and especially in DS group. It can be concluded
that SA improves drought tolerance in I. walleriana grown ex vitro probably by modulating the
activity of antioxidative enzymes and can be used as a drought-ameliorating agent.
Keywords:
drought; salicylic acid; antioxidative enzymesFunding / projects:
- Development and application of biotechnological methods for obtaining healthy plants of ornamental species (RS-MESTD-Technological Development (TD or TR)-31019)
- Development and application of protein markers for heat tolerance screening in potato (RS-MESTD-Technological Development (TD or TR)-31049)
In:
- Uzelac B, editor. Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia. Belgrade: Serbian Plant Physiology Society; Institute for Biological Research "Siniša Stanković"; Faculty of Biology; 2018. p. 73.