Phenolic compounds in ethanol extracts of different plant organs of Inula oculis-christi L.
2018
Аутори:
Gašić, UrošSkorić, Marijana
Živković, Suzana
Nestorović Živković, Jasmina
Filipović, Biljana
Todorović, Slađana
Tešević, Živojin
Остала ауторства
Uzelac, BrankaТип документа:
Конференцијски прилог (Објављена верзија)
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© 2018 by the Serbian Plant Physiology Society
Метаподаци
Приказ свих података о документуАпстракт:
Inula oculus-christi L. (family Asteraceae), grows naturally in Asia, Central Europe and the Balkan
Peninsula. Sesquiterpenoids, flavonoids and phenolics as major secondary metabolites contribute
to a variety of biological activities of this perennial species. In this research, an emphasis is given to the analysis of phenolic compounds in ethanol extracts of leaves, flowers, stems, rhizomes, and roots of I. oculus-christi. The content of total phenolics (TPC) and total flavonoids (TFC) in extracts of different plant parts of I. oculus-christi was determined. Significantly high TPC and TFC were observed in extract of flowers, while the extracts of stems and roots had the lowest content of phenolic compounds. UHPLC–LTQ/orbitrap/MS analysis was performed for separation, identification, and quantification of the major phenolic components in I. oculus-christi extracts. A total of 46 compounds detected belong to three structurally distinct groups: 1) hydroxycinnamic acid derivatives (14 compounds); 2) flavonoid glycosides (21 compounds); and 3) flavonoid aglycones (11 compounds). Dominant hydroxycinnamates were derivatives of caffeic acid, esterified with quinic or aldaric acids. The relative abundance of these compounds was higher in extracts of flowers, leaves and rhizomes. From the flavonoid glycoside group, flavone (nepetin, luteolin, apigenin, and chrysoeriol) and flavonol (quercetin, patuletin, kaempferol, isorhamnetin, and 3,3’-dimethylquercetin) derivatives were found in tested samples. Flavonoid aglycones were the most abundant compounds in extracts of flowers. Observed differences in phenolic content highlight the possibility of targeted collection and application of different I. oculus-christi extracts considering previously determined biological activities.
Кључне речи:
Inula oculus-christi; UHPLC-Orbitrap MS; Hydroxycinnamic acid derivatives; Flavonoid glycosides; Flavonoid aglyconesФинансирање / пројекти:
- Физиолошка, хемијска и молекуларна анализа диверзитета одабраних ретких и угрожених биљних врста у циљу еx ситу заштите и продукције биолошки активних једињења (RS-MESTD-Basic Research (BR or ON)-173024)
У:
- Uzelac B, editor. Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia. Belgrade: Serbian Plant Physiology Society; Institute for Biological Research "Siniša Stanković"; Faculty of Biology; 2018. p. 117-8.