Comparative study of antiproliferative potential of three different plant species
2019
Аутори:
Drakulić, DanijelaStojković, Dejan
Soković, Marina
Stevanović, Milena
Остала ауторства
Soković, MarinaТип документа:
Конференцијски прилог (Објављена верзија)
,
© 2019 Institute for Biological Research "Siniša Stanković", University of Belgrade
Метаподаци
Приказ свих података о документуАпстракт:
Cancer represents a heterogeneous group of diseases characterized by uncontrolled
proliferation of cells, tissue invasion and metastasis. Despite current therapeutic
strategies which include a combination of surgery, radiation and chemotherapy, cancer
is second leading cause of death worldwide next to cardiovascular diseases. Therefore,
the identification of new drugs with high sensitivity to cancer cells is warranted. The
anticancer properties of plants and their compounds have been shown since ancient
times and nowadays, there is a growing interest for identification of new plant-derived
anticancer agents.
This study was designed to analyze antiproliferative potential of three different plant
species, namely Ononis spinosa L. (Fabaceae), Anthriscus cerefolium (L.) Hoffm. (Apiaceae)
and Phlomis fruticosa L. (Lamiaceae). The aerial parts of plants were collected, dried
and the plant material was subjected to successive extraction with methanol and
then evaporated to dryness on a rotary vacuum evaporator. The obtained methanolic
extracts were used for further analysis. Cytotoxic effect of methanolic extracts on HaCaT
(spontaneously immortalized human keratinocyte cell line), MCF-7 (breast cancer cell
line), SiHa (cervical cancer cell line), A172 (glioblastoma cell line) and HepG2 (human
hepatocellular carcinoma cell line) cells was determined by crystal violet colorimetric
assay. Antiproliferative activity of methanolic extracts on glioblastoma cells was
evaluated by Ki67 immunostaining.
The results showed that plant extracts reduced viability of all tested cell lines. The
most active extract was the one from O. spinosa, indicating by the lowest IC50 value.
Ki67 immunostaining indicates antiproliferative activity of methanolic extracts on
glioblastoma cells.
Кључне речи:
Cancer; Plants; Methanoli; Extracts; Antiproliferative activity; KI-67Финансирање / пројекти:
- Карактеризација и примена метаболита гљива и утврђивање потенцијала нових биофунгицида (RS-MESTD-Basic Research (BR or ON)-173032)
- Проучавање сигналних путева и епигенетичких механизама укључених у контролу експресије хуманих SOX гена: даље расветљавање њихове улоге у одређивању судбине и диференцијацији ћелија (RS-MESTD-Basic Research (BR or ON)-173051)
У:
- Soković M, editor. Book of abstracts: The 2nd Balkans-China Mini-symposium on Natural Products and Drug Discovery; 2019 Apr 11-13; Belgrade, Serbia. Belgrade: Institute for Biological Research "Siniša Stanković", University of Belgrade; 2019. p 38