Table tomato leaves are a sustainable source of rutin and display antioxidant, antihemolytic and antimicrobial activity
2021
Аутори:
Añibarro-Ortega, MikelPinela, José
Ćirić, Ana
Rocha, Filomena
Barata, Ana Maria
Soković, Marina
Carvalho, Ana Maria
Ferreira, Isabel C. F. R.
Barros, Lillian
Остала ауторства
Barros, LillianCastañeda, Bruno Melgar
Shiraishi, Carlos Seiti Hurtado
Тип документа:
Конференцијски прилог (Објављена верзија)
,
© 2021 by the Instituto Politécnico de Bragança, Centro de Investigacao de Montanha
Метаподаци
Приказ свих података о документуАпстракт:
Table tomato (Solanum lycopersicum L.) is highly consumed worldwide and its annual production generates
a considerable amount of plant by-products [1]. Although tomatoes are well-known for their composition
in nutrients and bioactive compounds [2,3], little information is available in literature regarding tomato
leaves, probably because these by-products have no commercial value. Therefore, considering the enormous
production of this biomass and the current trends of circularity and sustainable development [3], this study
was carried out to evaluate the polyphenolic composition and bioactive properties of tomato leaves resulting
from the regeneration of table tomato landraces conserved ex-situ in the Portuguese Genebank (BPGV), in
Braga, Portugal. The tomato plant material resulting from pruning performed after the flowering season was
lyophilized and ground to a fine powder, used to prepare hydroethanolic extracts [4]. These extracts were
characterized for their composition in polyphenols (by HPLC-DAD-ESI/MSn
) and used to evaluate the
antioxidant (by DPPH•
scavenging capacity and reduction power), antihemolytic (using sheep erythrocytes
and AAPH as a free radical generator), and antimicrobial (tested against food-borne bacterial and fungal
strains by the serial microdilution and p-iodonitrotetrazolium violet colorimetric methods) activities [4].
The chromatographic analysis allowed identifying phenolic acids and flavonoids, with prevalence of
quercetin-3-O-rutinoside (rutin). The leaf extracts showed antioxidant activity, with EC50 values lower than
those previously reported for the respective tomato fruits, thus translating a higher activity [2]. The extracts
were also effective in some extent in protecting the erythrocytes from the oxidative hemolysis caused the
thermal decomposition of the free-radical initiator AAPH. Despite their low activity against the tested
microfungi, some extracts had ability to inhibit and kill some bacteria (including Salmonella typhimurium,
Listeria monocytogenes, Bacillus cereus, and Enterobacter cloacae) more effectively than the antibiotic
ampicillin. It was concluded that table tomato crop by-products can be used to produce rutin-rich extracts
with antioxidant and antibacterial activities for possible use in the agri-food sector as natural preservatives.
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200007 (Универзитет у Београду, Институт за биолошка истраживања 'Синиша Станковић') (RS-MESTD-inst-2020-200007)
У:
- Barros L, Castañeda BM, Shiraishi CSH, editors. 1st Natural products application: Health, Cosmetic and Food: book of abstracts; 2021 Feb 4-5; Online. Bragança, Portugal: Instituto Politécnico de Bragança (IPB); 2021. p. 73.