Biological activity of chicory extracts and compounds
2021
Аутори:
Stojković, DejanIvanov, Marija
Petrović, Jovana
Ćirić, Ana
Soković, Marina
Остала ауторства
Barros, LillianCastañeda, Bruno Melgar
Shiraishi, Carlos Seiti Hurtado
Тип документа:
Конференцијски прилог (Објављена верзија)
,
© 2021 by the Instituto Politécnico de Bragança, Centro de Investigacao de Montanha
Метаподаци
Приказ свих података о документуАпстракт:
Cichorium intybus L. (Asteraceae) or chicory is a leafy biennial or perennial plant, with a strong fleshy
taproot, that can grow up to 75 cm in length. Chicory has a long history being used as medicinal plant. The
major sesquiterpene lactones (STLs) of chicory belong to the class of guaianolide sesquiterpene lactones
and are commonly derived from a single sesquiterpene, germacrene A. The main STLs in chicory are
lactucin, lactucopicrin, 8-deoxylactucin and their oxalate and glucoside derivatives.
The aim of this study was to screen the different types of chicory extracts and terpene compounds for their
bioactivities and to understand better how the valuable currently unused part of chicory could be taken to
use.
Antimicrobial activity of terpene samples was tested via analysing the type of growth inhibition (biostatic
or biocidic) using modified microdilution method. For the determination of the antimicrobial activity of
terpene samples, a modified bioautographic method was used as well. Impact of extracts and compounds
on biofilm formation were determined on Candida albicans 475/15 and Pseudomonas aeruginosa (AMPR).
Cristal violet assay was used for the determination of antiproliferative effect, on human gingival fibroblasts
cells HGF-1 (ATCC® CRL-2014™).
It was observed that EtOAc extract of chicory root showed good antimicrobial potential against all tested
bacteria (MIC 0.75-3 mg/mL) except for P. aeruginosa (AMPR) strain, while water root extract did not
display antimicrobial effect with tested concentrations. Costunolide, lactucopicrin, 11β,13-dihydrolactucin,
compounds found in chicory roots, showed the most prominent effect against resistant strain of P.
aeruginosa. All tested standard compounds demonstrated very good antifungal activity against Candida
species (MIC 0.03-1.00 mg/mL). By using bioautographic method we have shown that none of the extract
fractions were active against Escherichia coli, while two fractions of EtOAc extract were active against P.
aeruginosa. EtOAc extract of chicory showed the most promising activity in inhibiting C. albicans biofilm
formation, while slightly lower activity was observed for compound parthenolide. Water extract did not
exhibit any cytotoxicity up to 1 mg/mL. On the other hand, EtOAc extract was cytotoxic to HGF-1 cell at
GI50(%) concentration of 0.750 mg/mL. As for the tested compounds, 11β-13-dihydrolactucin was the less
toxic to HGF-1 cells. All other compounds were toxic at the lowest concentration used
Финансирање / пројекти:
У:
- Barros L, Castañeda BM, Shiraishi CSH, editors. 1st Natural products application: Health, Cosmetic and Food: book of abstracts; 2021 Feb 4-5; Online. Bragança, Portugal: Instituto Politécnico de Bragança (IPB); 2021. p. 118.