Chicory fraction and 8-deoxylactucin inhibit growth and virulence traits of resistant Pseudomonas aeruginosa
2021
Authors:
Ćirić, AnaIvanov, Marija
Stojković, Dejan
Glamočlija, Jasmina
Kostić, Marina
Soković, Marina
Document Type:
Conference object (Published version)
,
© 2021 by the International Association of Food Nutrition and Safety
Metadata
Show full item recordAbstract:
Chicory (Cichorium intybus L.) is a plant widely cultivated for the inulin production. The
plant is underutilized regarding sesquiterpene lactones that might express potent
pharmacological activities, and are currently discharged as a byproduct. Our study aimed to
investigate both fraction and lactone antimicrobial potential and ability to reduce viability of
P. aeruginosa cells in biofilms (MTT assay), as well as their ability to produce
exopolysaccharides (Congo red binding assay), extracellular DNA and form biofilms on
urinary catheters. Chicory fraction and 8-deoxylactucin exhibited promising antibacterial
activity towards antibiotic resistant strain of P. aeruginosa (MIC 0.5 and 0.06 mg/mL,
respectively). Chicory fraction has reduced drastically (>50% inhibition with MIC of agent)
viability of P. aeruginosa cells in biofilm, while the activity of 8-deoxylactucin was less
profound (<30% inhibition). Likewise, application of chicory fraction in MIC concentration
has significantly limited the ability of P. aeruginosa cells to produce exopolysaccharides
(>40% inhibition) and eDNA (>60% inhibition), known biofilm resistance factors. Biofilms
formed on urinary catheters are challenging threat in hospital environments. Though, ability
of both chicory fraction and 8-deoxylactucin to reduce ability of P. aeruginosa to attach to
catheter surface should be further explored as promising novel antibacterial treatment since
application of both agents at their MIC concentrations has induced more than 50% inhibition.
Funding / projects:
In:
- 5th International Symposium on Phytochemicals in Medicine and Food: Abstracts; 2021 Aug 25 - Sep 1; Nanchang, China. International Association of Food Nutrition and Safety; 2021. p. 371.