Phenolics content and antioxidant activity of two Asplenium species: life cycle-specific patterns
2018
Authors:
Živković, SuzanaMaksimović, Vuk
Milutinović, Milica
Božunović, Jelena
Aničić, Neda
Šiler, Branislav
Božić, Dragana
Mišić, Danijela
Contributors
Soković, MarinaTešić, Živoslav
Document Type:
Conference object (Published version)
,
© 2018 by the University of Belgrade
Metadata
Show full item recordAbstract:
Historically, ferns have been an important source of drugs, food, and fibers. Nevertheless, very few works have been done on their phytochemical characterization. Moreover, the haploid gametophyte phase of ferns has, probably due to the fragile structure, microscopic dimensions, and difficult collection, only rarely been the subject of phytochemical investigations. With the aim to support the ethnopharmacological importance of Asplenium species, the present work focuses on evaluating the phenolic content and antioxidant properties of sporophytes and gametophytes of Asplenium adiantum-nigrum L. and A. ruta-muraria L, two fern species widely distributed in Europe. Methanol extracts of
gametophytes and sporophytes (fronds, rhizomes and roots) were subjected to UHPLC/DAD/(−)HESI-MS/MS analysis, and further to the evaluation of their scavenging capacities against ABTS•+ and DPPH•.
Phenolics content and antioxidant activity of analyzed Asplenium species showed species-specific and life cyclespecific patterns. Identified phenolic compounds belong to the classes of phenolic acids (hydroxybenzoic and hydroxycynnamic acids), flavonoids (flavan-3-ols, flavonols), and xanthones. Sporophytes of A. adiantum-nigrum are especially rich in xanthones (mangiferin and mangiferin glycoside), potent bioactive compounds. Besides xanthones, gametophytes contain significant amounts of flavan-3-ols (epigallocatechin and epigallocatechin gallate). Flavan-3-ols are also recognized as the major phenolic compounds in both A. ruta-muraria sporophytes (gallocatechin gallate and epigallocatechin gallate) and gametophytes (gallocatechin and catechin). The excellent antioxidant activity of A. adiantum-nigrum rhizomes and A. ruta-muraria fronds, against both DPPH and ABTS radicals, could be ascribed to the high content of xanthones and flavan-3-ols, respectively. The results of the present study candidate these two fern species as potent antioxidant agents, thus supporting their traditional use.
Funding / projects:
- Physiological, chemical and molecular analysis of the diversity of selected rare and endangered plant species and application of biotechnology for ex situ conservation and production of biologically active compounds (RS-MESTD-Basic Research (BR or ON)-173024)
- The membranes as sites of interaction between the intracellular and apoplastic environments: studies of the bioenergetics and signaling using biophysical and biochemical techniques. (RS-MESTD-Basic Research (BR or ON)-173040)
In:
- Soković M, Tešić Ž, editors. Unifood conference: Programme & Book of Abstracts; 2018 Oct 5-6; Belgrade, Serbia. Belgrade: University of Belgrade; 2018. BKH10/FQS10.