Arabidopsis EML histone readers are essential for seed development
2018
Аутори:
Milutinović, MilicaCoursey, Tami
Lindsey, Benson E III
Bisaro, M David
Grotewold, Erich
Brkljačić, Jelena M.
Тип документа:
Конференцијски прилог (Објављена верзија)
,
© 2018 by the Serbian Plant Physiology Society
Метаподаци
Приказ свих података о документуАпстракт:
Histone post-translational modifications (PTMs) and their recognition by histone readers, play
a major role in regulating gene expression. Despite extensive studies, there is a lack of systematic
knowledge of histone readers in plants. EMSY-like (EML) gene family was identified in Arabidopsis
thaliana (Arabidopsis) based on homology with maize RIF1. RIF1 and EML proteins share a unique
protein domain organization, characterized by the ENT and AGENET domains, found only in proteins with chromatin-associated functions. We demonstrated that EML1 and EML3 bind histone
H3 lysine 36 (H3K36), a PTM usually associated with active gene expression and that they are
localized in different compartments during seed development. To functionally characterize Arabidopsis EML proteins, we generated eml1-2, and eml3-4 homozygous single mutant, as well as
eml1-2 eml3-4 double mutant stocks. Consistent with their localization pattern, mutants in EML1
or EML3 showed delayed embryo development, while in the eml1-2 eml3-4 we observed a more
significant delay compared with its WT. In addition, combined loss of function of EML1 and EML3
displayed early seed abortion and also caused ovule abortion, suggestive of a synergistic effect.
Therefore, our preliminary results suggest that EML genes, specific histone mark readers in seeds,
have an essential role in seed development.
Кључне речи:
Arabidopsis thaliana; post-translational modifications; histone reader; seed developmentФинансирање / пројекти:
- Физиолошка, хемијска и молекуларна анализа диверзитета одабраних ретких и угрожених биљних врста у циљу еx ситу заштите и продукције биолошки активних једињења (RS-MESTD-Basic Research (BR or ON)-173024)
У:
- Uzelac B, editor. Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia. Belgrade: Serbian Plant Physiology Society; Institute for Biological Research "Siniša Stanković"; Faculty of Biology; 2018. p. 35.