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Arabidopsis EML histone readers are essential for seed development
dc.creator | Milutinović, Milica | |
dc.creator | Coursey, Tami | |
dc.creator | Lindsey, Benson E III | |
dc.creator | Bisaro, M David | |
dc.creator | Grotewold, Erich | |
dc.creator | Brkljačić, Jelena M. | |
dc.date.accessioned | 2021-09-17T12:18:15Z | |
dc.date.available | 2021-09-17T12:18:15Z | |
dc.date.issued | 2018 | |
dc.identifier.isbn | 978-86-912591-4-3 | |
dc.identifier.uri | https://radar.ibiss.bg.ac.rs/handle/123456789/4402 | |
dc.description.abstract | Histone post-translational modifications (PTMs) and their recognition by histone readers, play a major role in regulating gene expression. Despite extensive studies, there is a lack of systematic knowledge of histone readers in plants. EMSY-like (EML) gene family was identified in Arabidopsis thaliana (Arabidopsis) based on homology with maize RIF1. RIF1 and EML proteins share a unique protein domain organization, characterized by the ENT and AGENET domains, found only in proteins with chromatin-associated functions. We demonstrated that EML1 and EML3 bind histone H3 lysine 36 (H3K36), a PTM usually associated with active gene expression and that they are localized in different compartments during seed development. To functionally characterize Arabidopsis EML proteins, we generated eml1-2, and eml3-4 homozygous single mutant, as well as eml1-2 eml3-4 double mutant stocks. Consistent with their localization pattern, mutants in EML1 or EML3 showed delayed embryo development, while in the eml1-2 eml3-4 we observed a more significant delay compared with its WT. In addition, combined loss of function of EML1 and EML3 displayed early seed abortion and also caused ovule abortion, suggestive of a synergistic effect. Therefore, our preliminary results suggest that EML genes, specific histone mark readers in seeds, have an essential role in seed development. | sr |
dc.language.iso | en | sr |
dc.publisher | Belgrade: Serbian Plant Physiology Society | sr |
dc.relation | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173024/RS// | sr |
dc.rights | openAccess | sr |
dc.source | 3rd International Conference on Plant Biology and 22nd SPPS Meeting , 9-12 June 2018, Belgrade | sr |
dc.subject | Arabidopsis thaliana | sr |
dc.subject | post-translational modifications | sr |
dc.subject | histone reader | sr |
dc.subject | seed development | sr |
dc.title | Arabidopsis EML histone readers are essential for seed development | sr |
dc.type | conferenceObject | sr |
dc.rights.license | ARR | sr |
dcterms.abstract | Линдсеy, Бенсон Е ИИИ; Милутиновић, Милица; Цоурсеy, Тами; Бркљачић, Јелена; Бисаро, М Давид; Гротеwолд, Ерицх; Aрабидопсис ЕМЛ хистоне реадерс аре ессентиал фор сеед девелопмент; Aрабидопсис ЕМЛ хистоне реадерс аре ессентиал фор сеед девелопмент; | |
dc.rights.holder | © 2018 by the Serbian Plant Physiology Society | sr |
dc.description.other | Uzelac B, editor. Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia. Belgrade: Serbian Plant Physiology Society; Institute for Biological Research "Siniša Stanković"; Faculty of Biology; 2018. p. 35. | sr |
dc.citation.spage | 35 | |
dc.type.version | publishedVersion | sr |
dc.identifier.cobiss | 264415756 | |
dc.identifier.fulltext | https://radar.ibiss.bg.ac.rs/bitstream/id/9049/3-IntConfPlantBiology_2018-35.pdf | |
dc.identifier.rcub | https://hdl.handle.net/21.15107/rcub_ibiss_4402 |