Alteration of flower color in Viola cornuta cv. ‘Lutea Splendens’ through metabolic engineering of capsanthin/capsorubin synthesis
2021
Аутори:
Trajković, MilenaJevremović, Slađana
Dragićević, Milan
Simonović, Ana
Subotić, Angelina
Milošević, Snežana
Cingel, Aleksandar
Тип документа:
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт:
Flower color is an important characteristic that determines the commercial value of ornamental plants. The development of modern biotechnology methods such as genetic engineering
enables the creation of new flower colors that cannot be achieved with classical methods of hybridization or mutational breeding. This is the first report on the successful Agrobacterium-mediated genetic
transformation of Viola cornuta L. The hypocotyl explants of cv. “Lutea Splendens” variety with
yellow flowers were transformed with A. tumefaciens carrying empty pWBVec10a vector (Llccs−) or
pWBVec10a/CaMV 35S::Llccs::TNos vector (Llccs+) for capsanthin/capsorubin synthase gene (Llccs)
from tiger lily (Lilium lancifolium). A comparative study of shoot multiplication, rooting ability during
culture in vitro, as well as phenotypic characteristics of untransformed (control) and transgenic
Llccs− and Llccs+ plants during ex vitro growth and flowering is presented. Successful integration
of Llccs transgene allows the synthesis of red pigment capsanthin in petal cells that gives flowers
different shades of an orange/reddish color. We demonstrate that the ectopic expression of Llccs gene
in ornamental plants, such as V. cornuta “Lutea Splendens” could successfully be used to change
flower color from yellow to different shades of orange.
Кључне речи:
Genetic transformation; Agrobacterium tumefaciens; Capsanthin; Horned pansy; Flower color change; Capsanthin/capsorubin synthase; Carotenoid biosynthesisИзвор:
Horticulturae, 2021, 7, 9, 324-Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200007 (Универзитет у Београду, Институт за биолошка истраживања 'Синиша Станковић') (RS-MESTD-inst-2020-200007)