New Data on Organization and Spatial Localization of B-Chromosomes in Cell Nuclei of the Yellow-Necked Mouse Apodemus flavicollis
2021
Аутори:
Karamysheva, TatyanaRomanenko, Svetlana
Makunin, Alexey
Rajičić, Marija
Bogdanov, Alexey
Trifonov, Vladimir
Blagojević, Jelena
Vujošević, Mladen
Orishchenko, Konstantin
Rubtsov, Nikolay
Тип документа:
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт:
The gene composition, function and evolution of B-chromosomes (Bs) have been actively discussed in recent years. However, the additional genomic elements are still enigmatic. One of Bs mysteries is their spatial organization in the interphase nucleus. It is known that heterochromatic compartments are not randomly localized in a nucleus. The purpose of this work was to study the organization and three-dimensional spatial arrangement of Bs in the interphase nucleus. Using microdissection of Bs and autosome centromeric heterochromatic regions of the yellow-necked mouse (Apodemus flavicollis) we obtained DNA probes for further two-dimensional (2D)- and three-dimensional (3D)- fluorescence in situ hybridization (FISH) studies. Simultaneous in situ hybridization of obtained here B-specific DNA probes and autosomal C-positive pericentromeric region-specific probes further corroborated the previously stated hypothesis about the pseudoautosomal origin of the additional chromosomes of this species. Analysis of the spatial organization of the Bs demonstrated the peripheral location of B-specific chromatin within the interphase nucleus and feasible contact with the nuclear envelope (similarly to pericentromeric regions of autosomes and sex chromosomes). It is assumed that such interaction is essential for the regulation of nuclear architecture. It also points out that Bs may follow the same mechanism as sex chromosomes to avoid a meiotic checkpoint.
Кључне речи:
3D genome organization; B-chromosomes (Bs); Chromosome territories (CT); Confocal microscopy; Duplication clusters; Microdissection; Repetitive DNA; SpeciationИзвор:
Cells, 2021, 10, 7, 1819-Финансирање / пројекти:
- Russian Foundation for Basic Research (#19-015-00084a)
- Ministry of Science and Higher Education of the Russian Federation via IC&G SB RAS (#0259-2021-0011)
- Government basic research program No. 0088-2021-0019 (Koltzov Institute of Developmental Biology RAS, Moscow, Russia)
- Ministry of Science and Higher Education of the Russian Federation via the Novosibirsk State University (#2019-0546 (FSUS-2020-0040))
DOI: 10.3390/cells10071819
ISSN: 2073-4409
PubMed: 34359988
WoS: 000676482400001
Scopus: 2-s2.0-85114069231
URI
http://www.ncbi.nlm.nih.gov/pubmed/34359988http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC8305704
https://www.mdpi.com/2073-4409/10/7/1819
https://radar.ibiss.bg.ac.rs/handle/123456789/4453