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Diverzitet vrste Fraxinus ornus iz Srbije i Crne Gore procenjena RAPD metodom
Diversity of Fraxinus ornus from Serbia and Montenegro as revealed by RAPDs
dc.creator | Bojović, Srđan | |
dc.creator | Heizmann, Philippe | |
dc.creator | Dražić, Dragana | |
dc.creator | Kovačević, Dragan | |
dc.creator | Marin, Petar | |
dc.creator | Popović, Zorica | |
dc.creator | Matić, Rada | |
dc.creator | Jurc, Maja | |
dc.date.accessioned | 2015-08-28T10:26:51Z | |
dc.date.available | 2015-08-28T10:26:51Z | |
dc.date.issued | 2013 | |
dc.date.issued | 2013 | sr |
dc.identifier.issn | 0534-0012 | sr |
dc.identifier.other | Rad_konverzija_492 | sr |
dc.identifier.uri | https://radar.ibiss.bg.ac.rs/handle/123456789/447 | |
dc.description.abstract | PCR-RAPD markers revealed individual variation in F. ornus. A total of 122 fragments were amplified using 7 primers and of these 97 fragments were polymorphic. The percentage of polymorphic loci was between 53.3% and 74.6% with an average of 63.1%. The mean gene diversity for all populations was 0.30 and the mean Shannon's index was 0.44. Of the total genetic variation 87% was intra-population whilst 13% was inter-population. The Mantel test revealed significant correlation between genetic and geographical distance matrice. Results herein represent the first use of molecular genetic (DNA) markers to characterize genetic variation in F. ornus populations. The partition of total genetic variance indicates a relatively restricted population differentiation as expected in outcrossing species. Present and future information on genetic structure and variability in F. ornus needs to be incorporated into strategies for the preservation of genetic resources of tree species. | en |
dc.description.abstract | U radu su korišćeni PCR-RAPD markeri radi procene individualnih varijacija kod vrste F. ornus. Ukupno 122 fragmenta je amplificirano korišćenjem 7 prajmera, i među njima je bilo 97 polimorfnih fragmenata. Procenat polimorfnih lokusa se kretao između 53.3% i 74.6% sa prosečnom vrednošću od 63.1%. Srednji diverzitet gena za sve ispitivane populacije je iznosio 0.30, dok je srednji Shannon's index imao vrednost 0.44. Od totalne genetičke varijabilnosti 87% pripada intra-populacionoj varijabilnost, a 13% inter-populacionoj. Mantel test je pokazao značajne korelacije između matrica genetičke i geografske distance. Rezultati ovog rada predstavljaju prvu upotrebu molekularno genetičkih (DNA) markera u cilju određivanja genetičke varijabilnosti populacija F. ornus. Odnosi unutar ukupne genetičke varijabilnosti ukazuju na relativno ograničenu populacionu diferencijacije u odnosu na vrednosti koje su očekivane kod stranooplodne vrste. Na osnovu ovih kao i budućih informacija koje se odnose na genetičku strukturu i varijabilnost vrste F. ornus potrebno je kreirati strategije za očuvanje genetičkih resursa drvenastih vrsta. | sr |
dc.description.sponsorship | Projekat ministarstva br. 173011 | sr |
dc.language.iso | eng | sr |
dc.rights | openAccess | sr |
dc.source | Genetika | sr |
dc.subject | Manna ash | ENG |
dc.subject | genetic variance | ENG |
dc.subject | RAPD | ENG |
dc.title | Diverzitet vrste Fraxinus ornus iz Srbije i Crne Gore procenjena RAPD metodom | sr |
dc.title | Diversity of Fraxinus ornus from Serbia and Montenegro as revealed by RAPDs | en |
dc.type | article | sr |
dc.rights.license | ARR | |
dcterms.abstract | Хеизманн, Пхилиппе; Поповић, Зорица; Бојовић, Срђан; Јурц, Маја; Марин, Петар; Ковачевић, Драган; Матић, Рада; Дражић, Драгана; | |
dc.citation.issue | 1 | sr |
dc.citation.volume | 45 | sr |
dc.citation.spage | 51 | sr |
dc.citation.epage | 62 | sr |
dc.type.version | publishedVersion | sr |
dc.identifier.fulltext | https://radar.ibiss.bg.ac.rs//bitstream/id/589/Rad_konverzija_492.pdf | |
dc.citation.rank | M23 | |
dc.identifier.rcub | https://hdl.handle.net/21.15107/rcub_ibiss_447 |