Oxidative Stress Parameters in Goitrogen-Exposed Crested Newt Larvae (Triturus spp.): Arrested Metamorphosis.
2021
Аутори:
Gavrić, JelenaDespotović, Svetlana
Gavrilović, Branka
Radovanović, Tijana
Petrović, Tamara
Ajduković, Maja
Vučić, Tijana
Cvijanović, Milena
Faggio, Caterina
Prokić, Marko
Тип документа:
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт:
Thiourea is an established disruptor of thyroid hormone synthesis and is frequently used as an inhibitor of metamorphosis. The changes caused by thiourea can affect processes associated with the oxidative status of individuals (metabolic rate, the HPI axis, antioxidant system). We investigated the parameters of oxidative stress in crested newt (Triturus spp.) larvae during normal development in late larval stage 62 and newly metamorphosed individuals, and during thiourea-stimulated metamorphosis arrest in individuals exposed to low (0.05%) and high (0.1%) concentrations of thiourea. Both groups of crested newts exposed to thiourea retained their larval characteristics until the end of the experiment. The low activities of antioxidant enzymes and the high lipid peroxidation level pointed to increased oxidative stress in larvae at the beginning of stage 62 as compared to fully metamorphosed individuals. The activities of catalase (CAT) and glutathione-S-transferase (GST) and the concentration of sulfhydryl (SH) groups were significantly lower in larvae reared in aqueous solutions containing thiourea than in newly metamorphosed individuals. The high thiourea concentration (0.1%) affected the antioxidative parameters to the extent that oxidative damage could not be avoided, contrary to a lower concentration. Our results provide a first insight into the physiological adaptations of crested newts during normal development and simulated metamorphosis arrest.
Кључне речи:
Amphibians; Antioxidative compounds; Metamorphosis; Thiourea; Thyroid hormonesИзвор:
International Journal of Environmental Research and Public Health, 2021, 18, 18, 9653-Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200007 (Универзитет у Београду, Институт за биолошка истраживања 'Синиша Станковић') (RS-MESTD-inst-2020-200007)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200178 (Универзитет у Београду, Биолошки факултет) (RS-MESTD-inst-2020-200178)
DOI: 10.3390/ijerph18189653
ISSN: 1660-4601
PubMed: 34574576
WoS: 000699585300001
Scopus: 2-s2.0-85114663339
URI
https://www.mdpi.com/1660-4601/18/18/9653http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC8464833
https://radar.ibiss.bg.ac.rs/handle/123456789/4483