Hypothyroidism Intensifies Both Canonic and the De Novo Pathway of Peroxisomal Biogenesis in Rat Brown Adipocytes in a Time-Dependent Manner.
2021
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Аутори:
Aleksić, MarijaGolić, Igor
Kalezić, Anđelika
Janković, Aleksandra
Korać, Bato
Korać, Aleksandra
Тип документа:
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт:
Despite peroxisomes being important partners of mitochondria by carrying out fatty acid oxidation in brown adipocytes, no clear evidence concerning peroxisome origin and way(s) of biogenesis exists. Herein we used methimazole-induced hypothyroidism for 7, 15, and 21 days to study peroxisomal remodeling and origin in rat brown adipocytes. We found that peroxisomes originated via both canonic, and de novo pathways. Each pathway operates in euthyroid control and over the course of hypothyroidism, in a time-dependent manner. Hypothyroidism increased the peroxisomal number by 1.8-, 3.6- and 5.8-fold on days 7, 15, and 21. Peroxisomal presence, their distribution, and their degree of maturation were heterogeneous in brown adipocytes in a Harlequin-like manner, reflecting differences in their origin. The canonic pathway, through numerous dumbbell-like and "pearls on strings" structures, supported by high levels of Pex11β and Drp1, prevailed on day 7. The de novo pathway of peroxisomal biogenesis started on day 15 and became dominant by day 21. The transition of peroxisomal biogenesis from canonic to the de novo pathway was driven by increased levels of Pex19, PMP70, Pex5S, and Pex26 and characterized by numerous tubular structures. Furthermore, specific peroxisomal origin from mitochondria, regardless of thyroid status, indicates their mutual regulation in rat brown adipocytes.
Кључне речи:
Biogenesis pathway; Brown adipocyte; Hypothyroidism; Inter-organellar association; PeroxisomeИзвор:
Cells, 2021, 10, 9, 2248-Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200007 (Универзитет у Београду, Институт за биолошка истраживања 'Синиша Станковић') (RS-MESTD-inst-2020-200007)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200178 (Универзитет у Београду, Биолошки факултет) (RS-MESTD-inst-2020-200178)
- WARMED - Development of No-Based Approaches for Guided White Adipose Tissue Browning. Can We Tackle Metabolic Diseases By Heating Up/Cooling Down the Fat? (RS-ScienceFundRS-Promis-6066747)
DOI: 10.3390/cells10092248
ISSN: 2073-4409
PubMed: 34571897
WoS: 000699389700001
Scopus: 2-s2.0-85115886717
URI
https://www.mdpi.com/2073-4409/10/9/2248http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC8472630
https://radar.ibiss.bg.ac.rs/handle/123456789/4491