Changes in Photosynthetic Pigments Content in Non-Transformed and AtCKX Transgenic Centaury (Centaurium erythraea Rafn) Shoots Grown under Salt Stress In Vitro
2021
Аутори:
Trifunović-Momčilov, MilanaMilošević, Snežana
Marković, Marija
Đurić, Marija
Jevremović, Slađana
Dragićević, Ivana Č.
Subotić, Angelina
Тип документа:
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт:
The effects of graded sodium chloride (NaCl) concentrations (0-, 50-, 100-, 150-, and 200-
mM) on photosynthetic pigment contents in in vitro grown shoots of important medicinal plant
species (Centaurium erythraea Rafn) were investigated. Non-transformed, one AtCKX1 and two
AtCKX2 transgenic centaury lines, with altered cytokinin profiles, were used in this study. The chlorophyll
(Chl) and carotenoid contents differed in the non-transformed and transgenic lines. In general,
salinity significantly reduced the Chl a and Chl b contents in comparison to the NaCl-free medium.
The lowest Chl content was observed in AtCKX2 transgenic shoots grown on all the culture
media. The total carotenoid content was increased in shoots of non-transformed and both AtCKX2
transgenic lines grown in 50-mM NaCl. On the other hand, in concentrations ˃50-mM NaCl, the
total carotenoid content was decreased in all analysed centaury shoots. The Chl a/Chl b ratio in all
the shoots increased progressively in the graded NaCl concentrations. Contrarily, the addition of
NaCl in the culture medium reduced the Chl/carotenoid ratio in centaury shoots. Taken together,
the results of this study partly explained the mode of centaury plant adaptations to salt stress in
vitro. Thus, the results on centaury shoots confirmed that the determination of the photosynthetic
pigment contents can be a very useful non-destructive screening method in order to discriminate
susceptible and resistant plant species/lines to salt stress conditions.
Кључне речи:
Centaury; Salinity; Abiotic stress; AtCKX genes; Chlorophyll; CytokininsИзвор:
Agronomy, 2021, 11, 10, 2056-Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200007 (Универзитет у Београду, Институт за биолошка истраживања 'Синиша Станковић') (RS-MESTD-inst-2020-200007)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200178 (Универзитет у Београду, Биолошки факултет) (RS-MESTD-inst-2020-200178)
DOI: 10.3390/agronomy11102056
ISSN: 2073-4395