Efekti oktreotida na ACTH ćelije hipofize ženki pacova

Abstract

The effects of intracerebroventricular (i.c.v.) application of a somatostatin analogue, Octreotide, on growth of pituitary adrenocorticotropes (ACTH cells) were examined in adult female Wistar rats. The animals were subjected to i.c.v. administration of three 1.0μg doses of Octreotide dissolved in 10μ1 saline every second day. Controls were treated in the same way with the same volume of saline. ACTH-producing cells were studied using the peroxidase -antiperoxidase (PAP) immunohistochemical procedure. Octreotide treatment of rat females, significantly decreased (p<0.05) all morphometric parameters measured, i.e. volume of ACTH cells and their nuclei by 52% and 11 %, respectively, as compared to the controls. The volume densities were also significantly decreased (by 47%; p<0.05) in comparison with the corresponding controls. These findings suggest that centrally administered somatostatin analogue (Octreotide) is specifically involved in the control of growth and secretory activity of ACTH cells in female rats.

Ispitivani su efekti intracerebroventrikularno (i.c.v.) ubrizganog analoga somatostatina, Oktreotida, na rast adrenokortikalnih (ACTH) ćelija adenohipofize ženki Wistar pacova. Sve eksperimentalne životinje su primile tri 1μg doze Oktreotida rastvorenog u 10μL fiziološkog rastvora, svaki drugi dan. Kontrole su tretirane na isti način fiziološkim rastvorom. Pacovi su žrtvovani petog dana po poslednjoj primljenoj dozi Oktreotida. ACTH ćelije su imunocitohemijski bojene PAP metodom. Dobijeni rezultati pokazuju da su statistički značajno smanjeni svi morfometrijski parametri tretiranih ženki pacova u odnosu na odgovarajuću kontrolu. Zapremina ćelija je smanjena za 52%, jedara za 11%, a volumenska gustina za 47% (p<0.05) u poređenju sa odgovarajućom kontrolom. Na osnovu dobijenih rezultata maze se zaključiti za Oktreotid deluje inhibitorno na rast ACTH ćelija adenohipofize ženki pacova.