Efekat nutritivnog statusa na nivo akutnog faznog odgovora proteina kod ženki pacova

Abstract

In response to inflammation, infection and tissue injury, the sequence of events known as acute-phase (AP) response is induced and a number of plasma proteins, called acute-phase proteins (APPs) are synthesized in the liver as a part of the host defense mechanism. Any condition which impairs acute phase protein response (APPR) and hence organism's ability to restore homeostasis may limit its survival in various stress circumstances. Malnutrition is well-known causes of several metabolic, immune and neuroendocrine dysfunctions and this study was aimed at investigating its effect on the magnitude of normal APPR and in relation to turpentine injury. Malnutrition in female Wistar rats was induced by food restriction during six weeks period until they riched and kept body weight to 50% of that of ad libitum fed mates. Results of rocket immunoelectrophoresis revealed that malnutrition alone or in conjunction with turpentine was capable of elevating an expression of one of APPs, haptoglobin (Hp). Based on the studies of liver-specific regulatory proteins that bind to hormone regulatory elements of Hp gene and thus determine its expression we found that malnutrition probably modulated Hp response by changing the expression of active members of C/EBP, STAT and NF-kB transcription factor families.

U odgovoru na inflamaciju, infekciju i povredu tkiva, kao sastavni deo odbrambenih mehanizama u organizmu se pokreće sled događaja poznat kao akutno-fazni odgovor (AFO) u okviru koga se u jetri sintetiše grupa proteina plazme označena kao akutno fazni proteini (AFP). Svako stanje koje narušava akutno fazni proteinski odgovor organizma (AFPO), a time i njegovu sposobnost da ponovo uspostavi homeostazu može da deluje ograničavajuće na njegovo preživljavanje u uslovima različitih vrsta stresa. Izgladnjivanje je dobro poznat uzrok nekoliko metaboličkih, imunih i neuroendokrinih poremećaja te je ovaj rad imao za cilj da ispita uticaj neuhranjenosti na nivo normalnog AFPO-a i onog izazvanog akutnom povredom. Ženke pacova soja Wistar su izgladnjivane tokom perioda od šest nedelja sve do dostizanja telesne mase koja predstavlja 50% telesne mase ženki pacova koje su se u istom periodu hranile normalno. Rezultati "roket" imunoelektroforeze su pokazali da je izgladnjivanje, samo ili u sprezi sa terpentinskom povredom dovelo do uvecanja ekspresije jednog od AFP-a, haptoglobina (Hp). Izučavanjem za jetru specifičnih regulatornih proteina koji se vezuju za hormon regulatorne elemente gena Hp i time određuju nivo njegove ekspresije zaključili smo da izgladnjivanje najverovatnije moduliše odgovor Hp-a tako što menja ekspresiju aktivnih članova C/EBP, STAT and NF-kB familija transkripcionih faktora.