Ibogaine-Mediated ROS/Antioxidant Elevation in Isolated Rat Uterus Is β-Adrenergic Receptors and KATP Channels Mediated
2021
Аутори:
Tatalović, NikolaVidonja Uzelac, Teodora
Oreščanin Dušić, Zorana
Nikolić-Kokić, Aleksandra
Bresjanac, Mara
Blagojević, Duško
Остала ауторства
Taira, JunseiТип документа:
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт:
Ibogaine effects are mediated by cellular receptors, ATP depletion followed by ROS
production and antioxidant enzyme activity elevation in a dose and time dependent manner. Since
the role of KATP channels and β -adrenoceptors in ROS cellular circuit was established here we
explored their role in ibogaine pro-antioxidant effectiveness. Single dose of ibogaine (10 mg/L i.e.,
28.8 µmol/L) was applied to isolated rat uterus (spontaneous and Ca2+-stimulated) and contractility
and antioxidant enzymes activity were monitored during 4 h. Ibogaine increased amplitude and
frequency of spontaneous active uteri immediately after addition that was prevented by propranolol
( 1 and 2 adrenoceptors selective antagonists) and glibenclamide (KATP sensitive channels inhibitor;
only frequency) pre-treatment. In Ca2+-stimulated uteri, ibogaine decreased both amplitude and
frequency after 4 h. Pre-treatment with propranolol abolished ibogaine induced amplitude lowering,
while glibenclamide had no effect. In both types of active uterus, ibogaine induced a decrease in
SOD1 and an increase in CAT activity after 2 h. In Ca2+-stimulated uterus, there was also a decrease
of SOD2 activity after 2 h. After 4 h, SOD1 activity returned to the baseline level, but GSH-Px activity
increased. Pre-treatment with both propranolol and glibenclamide abolished observed changes of
antioxidant enzymes activity suggesting that ibogaine pro-antioxidative effectiveness is β -adrenergic
receptors and KATP channels mediated.
Кључне речи:
ibogaine; KATP channels; glibenclamide; β-adrenergic receptors; propranolol; antioxidative enzymes; superoxide dismutase; catalase; uterus; contractilityИзвор:
Antioxidants, 2021, 10, 11, 1792-Финансирање / пројекти:
- Slovenian ARRS Programme, grant number P3-0171
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200007 (Универзитет у Београду, Институт за биолошка истраживања 'Синиша Станковић') (RS-MESTD-inst-2020-200007)
DOI: 10.3390/antiox10111792
ISSN: 2076-3921