Fiziološko prilagođavanje larvi gubara, Lymantria dispar L., i žutotrbe, Euproctis chrysorrhoea L., (Lepidoptera) na delovanje fluorantena
Physiological adjustment of larvae of gypsy moth, Lymantria dispar L., and brown-tail moth, Euproctis chrysorrhoea L., (Lepidoptera) to the effects of fluoranthene
Abstract:
Fluoranthene is one of the most abundant polycyclic aromatic hydrocarbons, present in the leaves of various plants used for food by larvae of polyphagous insect species Lymantria dispar and Euproctis chrysorrhoea. The aim of dissertation was to gain insight into the patterns of physiological responses of L. dispar and E. chrysorrhoea larvae (Lepidoptera: Erebidae) to the presence of environmentally relevant concentrations of fluoranthene in food and to elucidate the mechanisms of defense against its harmful effects, which contributes to perceive the way they adjust to stressful environmental conditions. Chronic effects of fluoranthene (6.7 and 67 ng/g dry food weight) on L. dispar and E. chrysorrhoea larvae, originating from natural populations, were examined. Antioxidative and detoxification enzymes of midgut showed changes of activity under the influence of fluoranthene, with more pronounced responses in midgut tissue, and between-species differences in patterns of responses. Decreased expression of Hsp70 in the midgut, and different responses of Hsp70 in the brain of larvae were noticed. Significant changes of the activity of digestive enzymes and their isoforms in the midgut of larvae of both species exposed to fluoranthene were noticed. Exposure to fluorantene inhibited the activity of certain digestive enzymes and their isoforms of L. dispar larvae, and increased the activity of a number of enzymes and expression of isoforms of certain enzymes of E. chrysorrhoea larvae. Canonical discriminant analysis confirmed between-species differences in responses of antioxidative and digestive enzymes to the presence of fluoranthene in the diet. The allocation of energy resources to defense mechanisms affected the life history traits of treated larvae of both species.
Keywords:
Lymantria dispar L.; Euproctis chrysorrhoea L.; Hsp70; Fluorantene; Antioxidative enzymes; Detoxification enzymes; Digestive enzymes; Life history traitsSource:
Faculty of Biology, University of Belgrade, 2021, 1-118Funding / projects:
- The effects of magnetic fields and other environmental stressors on the physiological responses and behavior of different species (RS-MESTD-Basic Research (BR or ON)-173027)