The morphometric study of the effects of bisperoxovanadium (bpv(phen)) on neonatal drg neurons in culture
2021
Autori:
Todorović, NatašaStojadinović, Gordana
AlJamal, Kamal
Živić, Miroslav
Ostala autorstva
Marković, ZoranFilipović, Nenad
Tip dokumenta:
Konferencijski prilog (Objavljena verzija)
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© 2021 by the Institute for Information Technologies, University of Kragujevac
Metapodaci
Prikaz svih podataka o dokumentuApstrakt:
Unlike the neurons in the CNS, the peripheral neurons have certain intrinsic regenerative capacity.
After injury, peripheral neurons can switch to a cellular “state for growth”, with the expression profiles
similar to early developmental stages.
We looked at the changes of morphometric parameters induced in young peripheral neurons with
treatments that in adult neurons have growth-stimulatory effect. The experimental treatments compared
to control were: BpV (phen), an inhibitor of PTEN; and bFGF, basic fibroblast growth factor. The
neurite growth was measured on cultured dissociated dorsal root ganglia neonatal neurons fixed 24h
after treatment and immunostained with anti-neurofilament H (NF-H) phosphorylated antibody. FIJI
Simple Neurite Tracer was used for morphometry of individual neurons. 24h post treatment, compared
to control, total neurite length, length of primary and length of terminal branches, were increased by
bFGF but not by BpV treatment. In all measured parameters related to the degree of branching, BpV treated neurons had small dispersion of values and small mean values, reminiscent of literature data
stating that BpV treated neurons are elongated and less branched. However, the BpV did not have a
positive influence on neurite elongation, as was reported on adult neurons. In contrast, bFGF stimulated
elongation of young neurons in the manner similar to the effects described on the adult neurons.
Ključne reči:
Elongation; Simple Neurite Tracer; BranchingU:
- Marković Z, Filipović N, editors. Book of Proceedings: 1st International Conference on Chemo and BioInformatics, ICCBIKG 2021; 2021 Oct 26-27; Kragujevac, Serbia. Kragujevac, Serbia: Institute for Information Technologies, University of Kragujevac; 2021. p. 214-7.