Fructose diet ameliorates effects of macrophage migration inhibitory factor deficiency on prefrontal cortex inflammation, neural plasticity, and behavior in male mice.
2021
Аутори:
Vratarić, MilošŠenk, Vladimir
Bursać, Biljana
Gligorovska, Ljupka
Ignjatović, Đurđica
Kovačević, Sanja
Veličković, Nataša
Đorđević, Ana
Тип документа:
Чланак у часопису (Објављена верзија)
,
© 2021 International Union of Biochemistry and Molecular Biology.
Метаподаци
Приказ свих података о документуАпстракт:
Macrophage migration inhibitory factor (MIF) is a pro-inflammatory cytokine that represents a link between diet-induced inflammation and insulin resistance. Our aim was to examine whether fructose diet affects inflammation and insulin signaling in the prefrontal cortex (PFC) of Mif knockout mice (MIF-KO), and their possible link to neural plasticity and behavior. We analyzed nuclear factor κB (NF-κB) and glucocorticoid signaling, expression of F4/80, IL-1β, TNF-α, TLR-4, MyD88, arginase 1 (Arg-1), mannose receptor (Mrc-1), and leukemia inhibitory factor (Lif) to assess inflammation in the PFC of C57/BL6J and MIF-KO mice consuming 20% fructose solution for 9 weeks. Insulin receptor (IR), IRS-1 serine phosphorylations (307 and 1101) and activity of PKCα, Akt, GSK-3β and AMPKα were used to analyze insulin signaling. Brain-derived neurotrophic factor (BDNF) and insulin-like growth factor 1 (IGF-1) mRNA levels, together with synapthophysin and PSD-95 protein level and calcium calmodulin-dependent kinase 2 (CaMKII) activity, were used as plasticity markers. Behavior was examined in elevated plus maze, light dark box and novel object recognition test. The results showed concomitant increase of Tnf-α, Tlr-4, MyD88 and M2 microglia markers (Arg-1, Mrc-1, Lif) in the PFC of MIF-KO, paralleled with unchanged glucocorticoid and insulin signaling. Increase of BDNF and IGF-1 was paralleled with increased CaMKII activity, decreased PSD-95 protein level, anxiogenic behavior, and impaired memory in MIF-KO mice. Fructose feeding restored these parameters in the PFC to the control level and mitigated behavioral changes, suggesting that ameliorating effects of fructose on neuroinflammation and behavior depend on the presence of MIF.
Кључне речи:
Behavior; Fructose; Inflammation; Insulin resistance; Macrophage migration inhibitory factor; Neural plasticity; Prefrontal cortexИзвор:
BioFactors (Oxford, England), 2021Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200007 (Универзитет у Београду, Институт за биолошка истраживања 'Синиша Станковић') (RS-MESTD-inst-2020-200007)
Повезане информације:
- Друга верзија
https://radar.ibiss.bg.ac.rs/handle/123456789/4773
DOI: 10.1002/biof.1802
ISSN: 0951-6433
PubMed: 34767656
WoS: 000717722300001
Scopus: 2-s2.0-85118830262
URI
https://onlinelibrary.wiley.com/doi/10.1002/biof.1802http://www.ncbi.nlm.nih.gov/pubmed/34767656
http://radar.ibiss.bg.ac.rs/handle/123456789/4687