The dampening of lipid droplet oscillatory movement in nitrogen starved filamentous fungi by a low dose of mitochondrial respiration inhibitor
2021
Authors:
Pajić, TanjaŽivić, Miroslav
Rabasović, Mihailo D.
Krmpot, Aleksandar J.
Todorović, Nataša
Contributors
Marković, ZoranFilipović, Nenad
Document Type:
Conference object (Published version)
Metadata
Show full item recordAbstract:
Lipid droplets (LDs) are small mobile organelles conserved in all eukaryotic cells. We wanted to
test if the LD movement can be muffled by an incomplete inhibition of mitochondrial respiration,
induced by treating hyphae of filamentous fungus Phycomyces blakesleeanus with 0.5 mM sodium
azide. Nitrogen starved hyphae were used, in order to obtain LDs in larger sizes and numbers. The data
obtained unequivocally showed: 1. Sodium azide treatment dramatically reduces the LD velocity and
the distances LDs travel; 2. LDs in both controls and in azide-treated hyphae oscillate in a small
confined space instead of travelling through the cell; 3. Azide-treated LDs oscillate less frequently and
in smaller confinement than controls.
Keywords:
Sodium azide; Phycomyces blakesleeanus; In vivo microscopy; Nile RedIn:
- Marković Z, Filipović N, editors. Book of Proceedings: 1st International Conference on Chemo and BioInformatics, ICCBIKG 2021; 2021 Oct 26-27; Kragujevac, Serbia. Kragujevac, Serbia: Institute for Information Technologies, University of Kragujevac; 2021. p. 226-9.