Efekat tretmana estradiolom i kalcijumom na prolaktinske ćelije u acikličnih ženki pacova

Abstract

The effects of estradiol dipropionate (EDP) or calcium glucoheptonate (Ca) on the morphology and stereology of the PRL cells in 14-month-oldWistar female rats were studied. The animals were treated daily with EDP in the dose of 0.625 mg/kg b.w. or calcium glucoheptonate (Ca; 11.4 mg/kg b.w.) for two weeks. The controls were injected with vehicle alone by the same schedule. Mammotrophs (PRL cells) were immunocytochemically localized by the PAP method. Blood PRL concentration was determined by Delfia procedure. In animals treated with EDP the volume of both, PRL cells and their nuclei, as well as the volume densities were significantly (p<0.05) increased by 17%, 9% and 38%, respectively, in comparison with the controls. In animals treated with Ca all morphometric parameters were insignificantly (p>0.05) decreased compared to control rats. Serum concentration of PRL was significantly increased (p<0.05) by 17% after estradiol treatment, but in Ca-treated females this parameter was insignificantly (p>0.05) changed by 2% compared to controls. Based on these results, it can be concluded that EDP expresses a strong stimulatory effect on the morphology and function of pituitary PRL cells.

Ispitivani su efekti višekratnih doza estradiol dipropionata (EDP) ili kalcijum glukoheptonata (Ca) na morfološke i stereološke karakteristike mamotropnih (PRL) ćelija u acikličnih ženki pacova. Aciklične ženke su svakodnevno tokom dve nedelje dobijale 0,625 mg/kg tm EDP-a ili 11,4 mg/kg tm Ca. PRL ćelije su imunocitohemijski obeležavane PAP metodom. U životinja tretiranih sa EDP-om zapremina ćelija i njihovih jedara kao i volumenska gustina signifikantno (p<0,05) su bile povećane za 17%, 9% i 38% u poređenju sa kontrolama. U životinja tretiranih kalcijumom ispitivani morfometrijski parametri nisu bili značajno (p>0,05) promenjeni. Koncentracija PRL u serumu značajno (p<0,05) je bila povećana (za 17%) posle tretmana estradiolom dok tretman kalcijumom nije izazvao značajne promene (2% u odnosu na kontrole). Na osnovu dobijenih rezultata može se zaključ iti da EDP ima snažan stimulatorni efekat na PRL ćelije u hipofizi.