Effects of Remote Ischaemic Preconditioning on the Internal Thoracic Artery Nitric Oxide Synthase Isoforms in Patients Undergoing Coronary Artery Bypass Grafting.
2021
Authors:
Janković, AleksandraZakić, Tamara
Miličić, Miroslav
Unić-Stojanović, Dragana
Kalezić, Anđelika
Korać, Aleksandra
Jović, Miomir
Korać, Bato
Document Type:
Article (Published version)
Metadata
Show full item recordAbstract:
Remote ischaemic preconditioning (RIPC) is a medical procedure that consists of repeated brief periods of transient ischaemia and reperfusion of distant organs (limbs) with the ability to provide internal organ protection from ischaemia. Even though RIPC has been successfully applied in patients with myocardial infarction during coronary revascularization (surgery/percutaneous angioplasty), the underlying molecular mechanisms are yet to be clarified. Thus, our study aimed to determine the role of nitric oxide synthase (NOS) isoforms in RIPC-induced protection (3 × 5 min of forearm ischaemia with 5 min of reperfusion) of arterial graft in patients undergoing urgent coronary artery bypass grafting (CABG). We examined RIPC effects on specific expression and immunolocalization of three NOS isoforms - endothelial (eNOS), inducible (iNOS) and neuronal (nNOS) in patients' internal thoracic artery (ITA) used as a graft. We found that the application of RIPC protocol leads to an increased protein expression of eNOS, which was further confirmed with strong eNOS immunopositivity, especially in the endothelium and smooth muscle cells of ITA. The same analysis of two other NOS isoforms, iNOS and nNOS, showed no significant differences between patients undergoing CABG with or without RIPC. Our results demonstrate RIPC-induced upregulation of eNOS in human ITA, pointing to its significance in achieving protective phenotype on a systemic level with important implications for graft patency.
Keywords:
Coronary artery graft; Internal thoracic artery; Nitric oxide synthase isoforms; Remote ischaemic preconditioningSource:
Antioxidants (Basel, Switzerland), 2021, 10, 12, 1910-Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200007 (University of Belgrade, Institute for Biological Research 'Siniša Stanković') (RS-MESTD-inst-2020-200007)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200178 (University of Belgrade, Faculty of Biology) (RS-MESTD-inst-2020-200178)
DOI: 10.3390/antiox10121910
ISSN: 2076-3921
PubMed: 34943013
WoS: 000735476400001
Scopus: 2-s2.0-85120039838
URI
https://www.mdpi.com/2076-3921/10/12/1910http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC8750270
http://radar.ibiss.bg.ac.rs/handle/123456789/4740