Efekat L-NAME na AlCl3-indukovanu toksičnost u mozgu pacova

Abstract

The present experiment was carried out to determine the effectiveness of nitric oxide synthase (NOS) inhibitor (L-NAME) in elevating the toxicity of AlCl3 on nitrite concentration and acetylcholine esterase activity of Wistar rats. Animals were killed 10 min and 3 days after the treatment and the forebrain cortex and striatum were removed. The results show that AlCl3 exposure promotes oxidative stress in different neural areas. The biochemical changes observed in neuronal tissues show that aluminium acts as a pro-oxidant, while NOS inhibitor exerts as antioxidant action in AlCl3-treated animals. In the present study, active avoidance learning was significantly impaired after AlCl3 injection, while pretreatment with L-NAME prevented the behavioural deficits caused between the 8th and 12th day after intrahippocampal application of neurotoxin. Our data suggest that aluminium may cause learning and memory deficits, while the treatment with L-NAME may decrease the oxidative stress and prevent learning and memory deficits caused by AlCl3.

U eksperimentu je ispitivana efikasnost inhibitora azot oksid sintaze (NOS)- L-NAME na toksičnost AlCl3 i određivana koncentracija nitrita i aktivnost acetilholin esteraze kod Wistar pacova. Životinje su dekapitovane 10 minuta ili 3 dana nakon tretmana i izolovani su kora prednjeg mozga i strijatum. Rezultati ukazuju da AlCl3 pokreće oksidativni stres u različitim regionima mozga. Biohemijske promene opisane u neuronskom tkivu ukazuju da aluminijum deluje kao prooksidans, dok inhibitor NOS ima antioksidativno dejstvo kod životinja tretiranih AlCl3. Reakcija aktivnog izbegavanja je bila znatno poremećena nakon aplikacije AlCl3, dok se davanjem L-NAME sprečavaju poremećaji ponašanja uzrokovani između 8. i 12. dana posle intrahipokampusne primene neurotoksina. Naši rezultati ukazuju da aluminijum može dovesti do smetnji u procesima učenja i pamćenja, dok tretman sa L-NAME smanjuje oksidativni stres i sprečava promene u učenju i pamćenju uzrokovane AlCl3.