Volatile Organic Compound Composition and Glandular Trichome Characteristics of In Vitro Propagated Clinopodium pulegium (Rochel) Bräuchler: Effect of Carbon Source.
2022
Аутори:
Stojičić, DraganaTošić, Svetlana
Stojanović, Gordana
Zlatković, Bojan
Jovanović, Snežana
Budimir, Snežana
Uzelac, Branka
Тип документа:
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт:
Clinopodium pulegium (Rochel) Bräuchler (Lamiaceae) is an endangered species endemic to the Southern Carpathians. It is characterized by the production of high amounts of essential oils, which emit volatile organic compounds (VOCs) that have an essential role in biotic and abiotic stress responses and in plant-plant and plant-insect interactions. The present study was initiated to phytochemically examine the influence of different carbon sources in the nutrition medium on VOC emissions of micropropagated C. pulegium plants, using gas chromatography-mass spectrometry analysis of headspace VOCs. The volatile profiles were subjected to multivariate analysis with respect to the presence, concentration and type of carbon source in the nutrient medium. In addition, the effect of different carbohydrates on the density and size of the leaf glandular trichomes, the main structures involved in the emission of VOCs, was determined. A total of 19 VOCs, primarily belonging to mono- and sesquiterpenes previously described in plants, were tentatively identified. Six VOCs were produced at levels higher than 2% of the total VOC emission, dominated by pulegone, ß-pinene and menthone. Inclusion of the carbohydrates in the culture media affected the production of the main leaf trichome-associated volatile allelochemicals although the qualitative composition of the volatiles changed only slightly. Multivariate analysis showed that the concentration, rather than the carbohydrate type, influenced the VOC profile.
Кључне речи:
Clinopodium pulegium; GC-MS; Lamiaceae; VOC profile; Carbohydrates; Glandular trichomes; Headspace; Micropropagation; TerpenoidsИзвор:
Plants (Basel, Switzerland), 2022, 11, 2, 198-Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200007 (Универзитет у Београду, Институт за биолошка истраживања 'Синиша Станковић') (RS-MESTD-inst-2020-200007)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200124 (Универзитет у Нишу, Природно-математички факултет) (RS-MESTD-inst-2020-200124)
DOI: 10.3390/plants11020198
ISSN: 2223-7747
PubMed: 35050086
WoS: 000757011700001
Scopus: 2-s2.0-85122692027
URI
https://www.mdpi.com/2223-7747/11/2/198http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC8778064
http://radar.ibiss.bg.ac.rs/handle/123456789/4768