Mesenchymal Stem Cells From Mouse Hair Follicles Reduce Hypertrophic Scarring in a Murine Wound Healing Model
2022
Аутори:
Li, HanluoZiemer, Mirjana
Stojanović, Ivana D.
Saksida, Tamara
Maksimović-Ivanić, Danijela
Mijatović, Sanja
Đmura, Goran
Mićanović, Dragica
Koprivica, Ivan
Krajnović, Tamara
Drača, Dijana
Simon, Jan-Christoph
Lethaus, Bernd
Savković, Vuk
Тип документа:
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт:
Wound healing of acute full-thickness injuries and chronic non-healing ulcers leads to delayed wound closure, prolonged recovery period and hypertrophic scarring, generating a demand for an autologous cell therapy and a relevant pre-clinical research models for wound healing. In this study, an immunocompetent model for wound healing was employed using a syngeneic murine cell line of mesenchymal stem cells cultured from the mouse whisker hair follicle outer root sheath (named moMSCORS). moMSCORS were isolated using an air-liquid interface method, expanded in vitro and characterized according to the MSC definition criteria - cell viability, in vitro proliferation, MSC phenotype and multi-lineage differentiations. Moreover, upon applying moMSCORS in an in vivo full-thickness wound model in the syngeneic C57BL/6 mice, the treated wounds displayed different morphology to that of the untreated wound beds. Quantitative evaluation of angiogenesis, granulation and wound closure involving clinical scoring and software-based quantification indicated a lower degree of inflammation in the treated wounds. Histological staining of treated wounds by the means of H&E, Alcian Blue, PicroSirius Red and αSMA immune labelling showed lower cellularity, less collagen filaments as well as thinner dermal and epidermal layers compared with the untreated wounds, indicating a general reduction of hypertrophic scars. The decreased inflammation, accelerated wound closure and non-hypertrophic scarring, which were facilitated by moMSCORS, hereby address a common problem of hypertrophic scars and non-physiological tissue properties upon wound closure, and additionally offer an in vivo model for the autologous cell-based wound healing.
Кључне речи:
Mesenchymal stem cells; Hair follicles; Outer root sheath; Syngeneic immunocompetent model; Wound healing; Murine wound model; Hypertrophic scarringИзвор:
Stem Cell Reviews and Reports, 2022Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200007 (Универзитет у Београду, Институт за биолошка истраживања 'Синиша Станковић') (RS-MESTD-inst-2020-200007)
- Clinic of Cranial Maxillofacial Plastic Surgery, University Hospital Leipzig, Germany
- Saxon Ministry of Science and Fine Arts (SMWK) to Saxon Incubator for Clinical Translation (SIKT) to VS
- German Research Council (DFG) SFB TRR 67 B12 to VS and B3 to SF and JCS
- Science Fund of the Republic of Serbia, Serbian Science and Diaspora Collaboration Program: Knowledge Exchange Vouchers No. 6370516, Project T1DmCell
- Erasmus Academic Exchange Program (D LEIPZIG 01, 01-1189)
DOI: 10.1007/s12015-021-10288-7
ISSN: 2629-3269
PubMed: 35080748