Nrf2-dependent control of redox and metabolic profile in the skin of hibernating ground squirrel (Spermophilus citellus)
2021
Аутори:
Budnar, MartaZakić, Tamara
Ćirović, Duško
Kalezić, Anđelika
Korać, Aleksandra
Janković, Aleksandra
Korać, Bato
Тип документа:
Конференцијски прилог (Објављена верзија)
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© 2021 Published by Elsevier Inc.
Метаподаци
Приказ свих података о документуАпстракт:
Hibernators enter into an extreme state of prolonged torpor characterized by hypometabolism (over 95% metabolic suppression), significant suppression of vital functions, and hypothermia. Specific regulatory mechanisms control physiological processes and metabolic functions that allow hibernators to survive challenging environmental conditions. Skin is one of the most metabolically active organs with intense fluctuations in metabolic rates that are accompanied by adequate changes in the antioxidant status. However, there is little data concerning redox-metabolic-related changes in hibernators skin. This study aimed to investigate nuclear factor erythroid 2–related factor 2 (Nrf2)-dependent control of redox and metabolic responses in the skin (epidermis and dermis) of ground squirrel (Spermophilus citellus) in pre-hibernation, hibernation (torpor), and post-hibernation. To that end, we examined the protein expression of Nrf2, hypoxia-inducible factor 1 alpha (HIF-1α), and key antioxidant defense (AD) and metabolic enzymes. Our results showed no change in HIF-1α protein expression during circannual phases, indicating that skin is not hypoxic during hibernation. Moreover, Nrf2 was strongly elevated in hibernation and post-hibernation, accompanied by higher methionine sulfoxide reductase A protein expression in hibernation. Interestingly, in hibernation protein levels of most AD enzymes remain on pre-hibernation level, while post-hibernation was characterized by high expression of manganese superoxide dismutase and glutathione peroxidase, indicating a possible role of Nrf2 in preconditioning during hibernation. Indeed, such AD and Nrf2 increases correlated with upregulation of pyruvate dehydrogenase, citrate synthase, complex III of the mitochondrial electron transport chain, and fatty acid synthase in hibernation and post-hibernation, and with an additional increase in enzymes of mitochondrial and peroxisomal β-oxidation in post-hibernation. The results suggest that Nrf2 is involved in the regulation of complex antioxidant response concerning changes in metabolic activity in the skin of ground squirrel in hibernation and post-hibernation to maintain optimal conditions for surviving.
У:
- Free Radical Research Europe (SFRR-E): Annual Meeting Abstracts: “Redox biology in the 21st century: a new scientific discipline”; 2021 Jun 15-18; Belgrade, Serbia. Elsevier; 2021. p. S65. (Free Radical Biology and Medicine; Vol. 177; Suppl. 1).