Contact hypersensitivity to dinitrochlorobenzene induces systemic immunomodulatory effects that are strain-dependent
2015
Аутори:
Popov Aleksandrov, AleksandraMirkov, Ivana
Demenesku, Jelena
Ninkov, Marina
Mileusnić, Dina
Jovanovic, Jelena
Kataranovski, Dragan
Kataranovski, Milena
Тип документа:
Конференцијски прилог (Објављена верзија)
,
© 2015 by the European Federation of Immunological Societies
Метаподаци
Приказ свих података о документуАпстракт:
Introduction: Contact hypersensitivity (CHS) is animal model of allergic contact dermatitis, common skin inflammatory disease. Although mechanisms of CHS are much studied,
systemic effects in challenge phase are far less known.
Materials and methods: Parameters of adaptive and innate/inflammatory reaction were examined locally (ear skin cells) and systemically [peripheral blood mononuclear cells (PBMC)
and spleen cells] 24 hours after challenge, in two rat strains, Dark Agouti (DA) and Albino Oxford (AO), previously shown to differ in intensity of ear swelling response and draining
lymph node cell activity in CHS.
Results: While production of IL-17 by ear skin cells after challenge was increased in both rat strains (vs. controls), production of IFN-γ and CD8+ cell number was increased only in DA.
Activity relevant for innate immunity (CD11b+ skin cell number and NO production) was significantly increased only in DA. Production of proinflammatory cytokines TNF-α and IL-1β
by these cells was unchanged in both strains. Similarly to ear skin cells, IL-17 production by PBMC was increased in both strains while IFN-γ, and TNF-α, IL-1β and NO, only in DA. In
the spleen, however, only IFN-γ production was increased in both strains, while other parameters were variably affected in DA and AO.
Conclusion: In CHS reaction to dinitrochlorobenzene proinflammatory/effector activity detected locally in skin, coincides with PBMC activity (more pronounced in DA compared to AO
rats). Systemic effect was compartment dependent, given differential activity of spleen cells in two strains.
Кључне речи:
Contact hypersensitivity (CHS); Dark Agouti (DA) and Albino Oxford (AO) rats; Ear skin cells; Peripheral blood mononuclear cells (PBMC); Spleen cells; Dinitrochlorobenzene (DNCB)У:
- Abstract book: 4th European Congress of Immunology: ECI 2015; 2015 Sep 6-9, 2015; Vienna, Austria. European Federation of Immunological Societies; 2015. p. 130.