Oral cadmium intake and immune responses in the gut: intestinal inflammation and immune priming of mesenteric lymph nodes
2015
Аутори:
Ninkov, MarinaPopov Aleksandrov, Aleksandra
Demenesku, Jelena
Mirkov, Ivana
Mileusnić, Dina
Tolinacki, Maja
Zolotarevski, Lidija
Kataranovski, Dragan
Brceski, Ilija
Kataranovski, Milena
Тип документа:
Конференцијски прилог (Објављена верзија)
,
© 2015 by the European Federation of Immunological Societies
Метаподаци
Приказ свих података о документуАпстракт:
Introduction: Toxic metal cadmium (Cd) is important food and drinking water contaminant. The majority of ingested cadmium retained in the gastrointestinal tract (GIT) mucosa
which allocates GIT as its main target. Mechanisms of induction of intestinal inflammatory response are largely unknown.
Materials and Methods: Effect of subchronic (30 days) oral (in water) cadmium administration (5 ppm and 50 ppm) was examined in Dark Agouti (DA) and Albino Oxford (AO) rats.
Beside intestinal immune response, activity of draining mesenteric lymph node (MLN) cells, central place for induction of intestinal immune tolerance and local protective responses,
was evaluated.
Results: In both rat strains cadmium consumption resulted in reduction of bacteria (Lactobacillus strain), intestinal tissue damage, modulated antioxidant enzymes activity and
inflammation [increased proinflammatory cytokine (TNF, IL-1β, IFN-γ, IL-17) content in DA rats; increased TNF and IL-10 content in AO rats] in duodenal homogenates. Accumulation
of cadmium in MLN was followed by stress response [elevation of MLN glutathione and metallothionein mRNA levels] only in DA rats. Stimulation of both adaptive (proliferation, Th1
and Th17 cytokine response) and innate immune activities (NKG2D+, CD68+ cells, selected oxidative activities, IL-1β) in MLN was observed, more pronounced in DA compared to AO
rats.
Conclusions: Oral intake of cadmium resulted in intestinal damage, inflammation, and induction of proinflammatory milleu and innate effector cell activities in MLN. Cadmiuminduced proinflammatory responses in DA rats but discrete immune responses of AO rats imply strain-dependent effects of oral cadmium administration.
Кључне речи:
Oral cadmium intake; Intestinal inflammation; Mesenteric lymph nodesФинансирање / пројекти:
- Имуномодулаторни ефекти ксенобиотика и биотичких фактора животне средине на популације мишоликих глодара (RS-MESTD-Basic Research (BR or ON)-173039)
У:
- Abstract book: 4th European Congress of Immunology: ECI 2015; 2015 Sep 6-9, 2015; Vienna, Austria. European Federation of Immunological Societies; 2015. p. 486.