Immunotoxicology of cadmium: Insight from acute intraperitoneal and intermediate period of oral exposure of rats
2015
Аутори:
Demenesku, JelenaNinkov, Marina
Popov Aleksandrov, Aleksandra
Mirkov, Ivana
Mileusnić, Dina
Zolotarevski, Lidija
Tolinački, Maja
Kataranovski, Dragan
Brceski, Ilija
Kataranovski, Milena
Тип документа:
Конференцијски прилог (Објављена верзија)
,
© 2015 by the Department of Biomedical Sciences-Histology of the University of Sassari
Метаподаци
Приказ свих података о документуАпстракт:
Conflicting data exist concerning cadmium
effects on immune system depending on the
experimental model, exposure or tissue/activity
examined. In this study immunotoxicity of acute
intraperitoneal and oral cadmium
administration was investigated in rats. The
use of the inflammation-prone inbred Dark
Agouti (DA) and less reactive Albino Oxford
(AO) rats showed differential (immune activityrelated and/or strain-related) effects of
cadmium (1 mg of Cd/kg, i.p.) on spleen
immune responses. A decrease in ConAinduced proliferation (related to altered spleen
cells responsiveness to IL-2) and of IFN-γ
(independently of IL-4 and IL-10) was more
pronounced in DA rats. Increased innate
immunity splenocyte activity (granulocyte
CD11b+ cells, iNOS mRNA and NO
production, myeloperoxidase MPO activity, IL1β mRNA and IL-1β protein product levels)
were observed in both strains (some of them
more pronounced in DA rats), while a decrease
in respiratory burst (dihydrorhodamine/DHR
oxidation) was similar. 30-day oral intake of 5
ppm and 50 ppm of cadmium by DA rats
resulted in reduction of some probiotic bacteria,
villous damage and intestinal inflammation
[(increased levels of High Mobility Group
Box1/HMGB1, antioxidant enzyme (superoxide
dismutase/SOD and catalase/CAT) activity and
proinflammatory cytokine (TNF, IL-1β, IFN-γ,
IL-17) in gut homogenates]. Stimulation of both
adaptive (increased cellularity, proliferation,
IFN-γ and IL-17cytokine responses) as well as
innate immune activity (increases in numbers
of NK cells and M1-like macrophages,
oxidative cell activities, IL-1β) of gut draining
(mesenteric) lymph nodes was associated with
decreased or unchanged antiinflammatory
cytokine (IL-10) cell response. Differential
(immunosuppressive and immunostimulatory)
effects noted in the same tissue (spleen)
should be taken into account when exploring
immunotoxicity of this metal. Stimulation of gut
immune responses imply dietary cadmium as
health risk factor.
Кључне речи:
Oral cadmium exposure; Intraperitoneal cadmium exposure; Dark Agouti (DA) and Abino Oxford (AO) rats; Spleen immune responsesФинансирање / пројекти:
- Имуномодулаторни ефекти ксенобиотика и биотичких фактора животне средине на популације мишоликих глодара (RS-MESTD-Basic Research (BR or ON)-173039)
У:
- II Cadmium Symposium 2015; 2015 Jun 25-27; Sassari, Italy. 2015. p. 31.