Subchronic oral intake of low cadmium doses affects intestinal immune responses in rats
2015
Аутори:
Ninkov, MarinaPopov Aleksandrov, Aleksandra
Demenesku, Jelena
Mirkov, Ivana
Mileusnić, Dina
Grigorov, Ilijana
Petrović, Anja
Zolotarevski, Lidija
Nikolic, Milica
Kataranovski, Milena
Остала ауторства
Lukić, MiodragJonjic, Stipan
Nikolich-Zugich, Janko
Тип документа:
Конференцијски прилог (Објављена верзија)
,
© 2015 by the Immunological Society of Serbia
Метаподаци
Приказ свих података о документуАпстракт:
Gastrointestinal (GI) tract is one of the main targets of cadmium (Cd),
important food and drinking water contaminant. In this study, the effect of
subchronic (30 days) oral (in drinking water) intake of environmentally
relevant doses of cadmium (5µg/ml and 50µg/ml) on intestinal [(tissue of
duodenum and mesenteric lymph node (mLN) cells)] was examined in
Dark Agouti (DA) rats. Atomic absorption spectrophotometry (AAS)
analysis revealed significant cadmium load in duodenum,which was
associated with changes of both intestinal bacterial load and composition
(Denaturing Gradient Gel Electrophoresis/DGGE).Shortening of villi,
damage to epithelium, increases in goblet-like vacuoles and mononuclear
cell infiltration in lamina propria were histologically evident at both
cadmium doses, with massive necrosis at higher Cd dose (judging by High
Mobility Group Box-1/HMGB-1 Western blot analysis).Increased levels of
proinflammatory cytokines (IL-1β, IFN-γ, IL-17) were observed at both Cd
doses (TNFα at higher dose solely). Cadmium administration affected
draining lymph nodes as well, judging by signs of stress response (drop of
cellular reduced glutathione/GSH at higher dose, rise of
metallothionein/MT mRNA at both doses).Increased cellularity of mLN
was observed at higher Cd dose, but with no changes in proliferative
responses. Intake of both Cd doses resulted in higher (compared to controls)
levels of IFN-γ and IL-17 mRNA as well as increased mLN cell
responsiveness to ConA stimulation.Significant increases in numbers of
CD68+ cells and stimulation of innate immune activities (numbers of
dihydrorhodamine/DHR+ cells and intracellular myeloperoxidase/MPO+
cells, LPS-stimulated nitric oxide/NO production and ex vivo IL-1β
expression) were observed at higher dose of cadmium.Proinflammatory
effects of cadmium might have resulted from changes in gut microbiota and
tissue damage.Interactions of this metal with intestinal immune system
should be taken into account when assessing dietary cadmium as health risk
factor.
Кључне речи:
Cadmium; Dark Agouti (DA) rats; Denaturing Gradient Gel Electrophoresis/DGGE; Intestinal immune systemФинансирање / пројекти:
- Имуномодулаторни ефекти ксенобиотика и биотичких фактора животне средине на популације мишоликих глодара (RS-MESTD-Basic Research (BR or ON)-173039)
У:
- Lukić M, Jonjic S, Nikolich-Zugich J, editors. 3rd Belgrade EFIS symposium on Immunoregulation: Immunity, Infection, Autoimmunity and Aging; 2015 May 24-27; Arandjelovac, Serbia. Belgrade: Immunological Society of Serbia; 2015. p. 48.