Differential strain impact on immune reactivity: insights from regional immune responses in rats
Diferencijalni uticaj soja na imunsku reaktivnost: uvid iz regionalnih imunskih odgovora kod pacova
2016
Аутори:
Ninkov, MarinaPopov Aleksandrov, Aleksandra
Mirkov, Ivana
Demenesku, Jelena
Brceski, Ilija
Tolinacki, Maja
Jovanovic, Sofija
Mileusnić, Dina
Kataranovski, Dragan
Kataranovski, Milena
Тип документа:
Конференцијски прилог (Објављена верзија)
,
© 2016 by the Immunological Society of Serbia
Метаподаци
Приказ свих података о документуАпстракт:
Our previous research showed a higher reactivity of DA (compared to AO rats) to antigens that cause skin and lung inflammatory reactions. In order to examine the effect of strains on the immune responses of other regions, the effect of oral (in drinking water, 30 days) intake of cadmium, a known food and water contaminant, on the intestinal immune response of AO and DA rats was analyzed. Despite similar amounts of cadmium deposited in the intestines of both strains, the reduction in Lactobacillus (important for maintaining immune homeostasis in the intestine) and tissue damage (histologically and according to the marker of tissue necrosis, HMGB-1) was more pronounced in DA rats.Changes, including the activity of antioxidant defense enzymes (superoxide dismutase and catalase), increased concentrations of IFN-γ and IL-17, and no changes in IL-10, which were detected in intestinal homogenates only in DA strains, indicate a more intense intestinal inflammatory reaction. compared to AO strain. The same concentrations of cadmium detected in the main draining (mesenteric) lymph nodes (MLC) led to the induction of mRNA for metal-binding redox proteins (metallothioneins, MT) only in DA rats. The presence of a proinflammatory cytokine response (protein products and mRNA) of MLC cells, detected predominantly in DA strains, indicates a more pronounced induction of cells that produce these cytokines.Increased cell proliferation and oxidative activity of MLC cells, as well as the number of CD68 +, NKG2D + and CD11b + cells only in DA rats, with a differential change in IL-10 (decrease in DA, increase in AO) emphasizes the inflammatory character of MLC rat microenvironment of this strain. The absence of similar changes in the spleen (at the same tissue load of cadmium as MLC) indicates the influence of damaged intestinal tissue on the activity of regional lymph nodes, and the more intense response of MLC DA soy reflects greater efforts to prevent systemic immune response to changes in intestinal homeostasis.
Кључне речи:
Dark Agouti (DA) and Albino Oxford (AO) rats; Oral cadmium exposure; Intestinal immune responseФинансирање / пројекти:
- Имуномодулаторни ефекти ксенобиотика и биотичких фактора животне средине на популације мишоликих глодара (RS-MESTD-Basic Research (BR or ON)-173039)
У:
- Knjiga apstrakata: VII Naučni sastanak Društva imunologa Srbije; 2016 Apr 27-28; Belgrade, Serbia. Belgrade: Immunological Society of Serbia; 2016. p. 10.