Influence of strain on the innate-immune reactions in rats

Abstract

Our previous studies have shown poorer activity / absence of cellular immune response in AO compared to DA rats. Given the scarcity of data on innate immunity reactions in these two strains, the aim of this study was to obtain initial data on this type of immune activity. Using a model of subcutaneously implanted polyvinyl sponges, the basic aspects of neutrophil leukocyte activity important for the response to A. fumigatus were examined.Although a higher number of neutrophil leukocytes of AO rats migrate to fungal-free sponges (group K), the yield of cells from fungal sponges (group A) is the same in both strains, while CD11b cell expression is lower in both AO groups compared to DA rats. . Despite the higher oxidative activity of cells from group AO compared to DA, NBT reduction is the same (spontaneous) or lower (after stimulation with PMA) in group A compared to K in AO, and the production of myeloperoxidase (MPO) and NO is lower .In DA rats, the presence of the fungus leads to an increase in the activity of A cells in relation to group K. Molecular identification of the bacterial composition of the lung microbiota under conditions of infection with A. fumigatus established the presence of Staphylococcus epidermidis and Veillonella cavie only in AO, and Streptococcus salivarius and Propionibacterium acne only in DA rats. The presence of different doses of S. epidermidis (SE) in the sponge with A led to inhibition of MPO production in both strains. Soybean differences were observed depending on the dose of SE and / or the test activity. The lowest dose leads to inhibition of NBT reduction in AO, but stimulation in DA, while higher doses of SE stimulate the response in both strains.The low concentration of the bacterium has no effect on the production of NO in AO, but higher doses stimulate it, in contrast to the inhibition in DA at all doses of SE. The presented results indicate the existence of strain differences in the innate-immune response of rats, as well as that they may depend on the type of activity examined.