(Bio)Chemical characterization of moss Hypnum cupressiforme extracts as potential immunomodulators
2021
Аутори:
Oalde, Mariana M.Mandić, Marija R.
Sabovljević, Aneta D.
Sabovljević, Marko S.
Gašić, Uroš
Duletić Laušević, Sonja N.
Božić, Bojan D.
Božić Nedeljković, Biljana D.
Тип документа:
Конференцијски прилог (Објављена верзија)
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© 2021 The Authors. European Journal of Immunology published by Wiley-VCH GmbH
Метаподаци
Приказ свих података о документуАпстракт:
In this study, we aimed to examine the chemical composition and biochemical activities (antioxidant, antidiabetic, and antineurodegenerative) of seasonal aspects (spring, summer, autumn) of moss Hypnum cupressiforme extracts. The extracts were prepared using Soxhlet extractor. Chemical characterization of extracts was performed via spectrophotometric assays and LC‐MS. The antioxidant activity of the extracts was determined by DPPH (2,2‐diphenyl‐1‐picryl‐hydrazyl‐hydrate), total reduction power, and β‐carotene bleaching assays. Extracts were analyzed for their inhibitory activity on α‐glucosidase, α‐amylase, acetylcholinesterase, and tyrosinase. The chemical analysis of the moss extracts revealed the presence of flavonoids, phenolic acids, and triterpenoids. The highest concentration of these compounds was found in the moss from summer season, and was significantly higher in comparison to the spring and autumn aspects. Major compounds identified by LC‐MS in H. cupressiforme extracts were kaempferol, p‐hydroxybenzoic, protocatechuic, p‐coumaric, gallic, and caffeic acid. According to biochemical assays investigated extracts exhibited significant antineurodegenerative potential, evaluated by the capacity to inhibit acetylcholinesterase and tyrosinase. Among the examined aspects, summer and autumn were found particularly efficient, especially at the lowest tested concentrations. Regarding β‐carotene bleaching test, the summer aspect showed the best activity at the highest concentration, while the spring aspect was found more efficient at the lowest investigated concentration. The obtained data suggest that the most favorable season in terms of chemical composition and biochemical characteristics of moss H. cupressiforme is summer. This moss is a promising source of biologically active compounds that may be useful in the treatment of various pathological conditions.
Кључне речи:
Immune regulation and therapy; immunopharmacology; inflammatory diseaseУ:
- Abstracts of ECI 2021: 6th European Congress of Immunology; 2021 Sep 1-4; Virtual Meeting. John Wiley and Sons; 2021. p. 296. (European Journal of Immunology; Vol. 51; No. S1).