Immunomodulatory properties of extracts of moss Hypnum cupressiforme from various seasons
2021
Аутори:
Mandić, Marija R.Oalđe, Mariana M.
Lunić, Tanja M.
Sabovljević, Aneta D.
Sabovljević, Marko S.
Gašić, Uroš
Duletić Laušević, Sonja N.
Božić, Bojan D.
Božić Nedeljković, Biljana D.
Тип документа:
Конференцијски прилог (Објављена верзија)
,
© 2021 The Authors. European Journal of Immunology published by Wiley-VCH GmbH
Метаподаци
Приказ свих података о документуАпстракт:
‚In this study, we aimed to examine the immunomodulatory potential (anti‐neuroinflammatory/neuroprotective and antitumor activities) of various seasonal aspects (spring, summer,
autumn) of moss Hypnum cupressiforme extracts. Moss extracts have been obtained using solvents of different polarities and their mixtures applying Soxhlet extractor. Activities
of the extracts were tested on cell lines MRC‐5, BV2, SH‐SY5Y, HCT‐116, and MDA‐MB‐231, for potential anti‐neuroinflammatory and antitumor activities. The impact of extracts on
cell viability/metabolic activity was measured by MTT assay, while the influence on the production of ROS and NO was determined using NBT and Griess assays, respectively. The
biocompatibility of extracts was confirmed using MRC‐5 cells. Extracts of moss from spring season have shown significant antiproliferative activity against MDA‐MB‐231 cell line
(inhibition rate ~50%), as well as anti‐neuroinflammatory activity. Furthermore, the effects of seasonal variation (spring, summer, autumn) on the immunomodulatory potential of
ethyl‐acetate extract were examined. Summer aspect of moss has led to the greatest reduction of ROS production, while autumn aspect had the greatest impact on the reduction of NO
production by LPS‐activated BV2 cells. Additionally, the supernatant‐transfer model proved that treatment with moss extracts from summer and autumn seasons of LPS‐activated BV2
cells exhibit neuroprotective activity towards SH‐SY5Y neurons. The obtained data suggest that extracts from moss H. cupressiforme possess significant immunomodulatory potential
evaluated in vitro, which may be useful in conditions such as Alzheimer’s disease, Parkinson’s disease, and breast cancer. In terms of anti‐neuroinflammatory/neuroprotective activity,
summer and autumn were characterized as the most favorable seasons.
Кључне речи:
Cancer immunology; immunotherapy; inflammatory diseaseУ:
- Abstracts of ECI 2021: 6th European Congress of Immunology; 2021 Sep 1-4; Virtual Meeting. John Wiley and Sons; 2021. p. 296. (European Journal of Immunology; Vol. 51; No. S1).