Evaluation of kanamycin and cefotaxime effects on proliferation, morphology and germination rate of somatic embryos in Centaurium erythraea Rafn
2021
Аутори:
Ćuković, KatarinaTodorović, Slađana
Dragićević, Milan
Simonović, Ana
Bogdanović, Milica
Остала ауторства
Kovačević, DušanТип документа:
Конференцијски прилог (Објављена верзија)
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© 2021 by the University of East Sarajevo: Faculty of Agriculture, Faculty of Agriculture
Метаподаци
Приказ свих података о документуАпстракт:
Centaurium erythraea Rafn is medically significant plant with a great potential in treating multiple gastrointestinal tract diseases. Although widely spread, C. erythraea is now listed as endangered species due to extensive exploitation, so there is a need to deepen knowledge of existing and developnew in vitro techniques for its mass propagation. Somatic embryogenesis (SE) is the most effective way for centaury in vitro regeneration. In addition to possessing great multiplication rate, regeneration via SE is also convenient for genetic transformation since somatic embryos offer genetically uniform starting material with less somaclonal variability. Furthermore, the ability of somatic embryos to undergo secondary SE, a process in which new somatic embryos are initiated from somatic embryos, makes them a suitable target tissue for transformation. We have recently established secondary SE in C. erythraea for the first time and our next step is to develop a transformation method using somatic embryos as starting material. Choice of the correct type and optimal concentration of decontamination and selection antibiotics is crucial in order to obtaina high germination rate and normal morphology of somatic embryos as a prerequisite for successful transformation. Therefore, we evaluated antibiotic sensitivity of untransformed somatic embryos, using different concentrations of cefotaxime and kanamycin as decontamination and selection antibiotics, respectively, and appropriate concentrations were determined. These conclusions were furthermore verified by visual observations of secondary somatic embryos number, their morphology as well as germination rate of embryos grown on media containing various antibiotics concentrations.
Кључне речи:
Centaurium erythraea; secondary somatic embryogenesis; Agrobacterium-mediated transformationФинансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200007 (Универзитет у Београду, Институт за биолошка истраживања 'Синиша Станковић') (RS-MESTD-inst-2020-200007)
У:
- Kovačević D, editor. Book of Abstracts: XII International Scientific Agriculture Symposium "Agrosym 2021"; 2021 Oct 7-10; Jahorina, Bosnia and Herzegovina. East Sarajevo: Faculty of Agriculture; 2021. p. 215.