Some blood parameters and antioxidant defense enzyme activities in the liver of carps (cyprinus carpio l) Under acute hypoxic conditions

Abstract

Carps (Cyprinus carpio L) were acclimated to oxygen concentration of 7.31 mg/L water (100%)-controls (C). Two experimental groups were exposed to a progressive reduction of O2 to 4.10 mg/L (56±1%) during 1 hour and 15 minutes (HI) and 1.95 mg/L (26±1%) during 2 hours (H2). In all examined groups haematological values: red blood cells (RBCs) count, haematocrite value (Htc), haemoglobin (Hb), glucose and lipid peroxide (LP) concentrations were studied. The antioxidant defense enzyme activities such as: Superoxide dismutase (SOD), catalase (CAT), glutathione-S-transferase (GST), glutathione peroxidase (GSH-Px) and glutathione reductase (GR) were also determined in the liver of carps. The acute hypoxia (HI) significantly increased the number of RBCs, Hb concentration and Htc value as well as LP concentration, whereas a significant decrease of glucose concentration was observed when compared with control fish. The activities of SOD, CAT GSH-Px, and GST in the liver were significantly increased in both experimental groups of fish (HI and H2) in respect to the controls. Liver GR activity was significantly increased only in HI group of carps.