Unraveling the adaptive significance of mitochondrial genome variability of Drosophila obscura
2021
Authors:
Erić, PavlePatenković, Aleksandra
Erić, Katarina
Tanasković, Marija
Davidović, Slobodan
Rakić, Mina
Savić Veselinović, Marija
Stamenković-Radak, Marina
Jelić, Mihailo
Document Type:
Conference object (Published version)
Metadata
Show full item recordAbstract:
Drosophila obscura is a very common fruit fly inhabiting European forests. This species has
a large number of mitochondrial haplotypes of Cyt b gene. We used experimental lines of D. obscura
to test the adaptive significance of intra -population variability of the mitochondrial genome
(mtDNA) and selective forces that maintain it. We chose three isofemale lines with distinct mitochondrial haplotypes of Cyt b gene from each of the two populations sampled in Serbia. Using backcrossing, we created nine experimental lines for each population with all combinations of mtDNA
haplotypes and nuclear genetic backgrounds (nuDNA). Individuals of both sexes were tested separately for desiccation resistance at two temperatures. Cox proportional hazards model, with four
factors: mtDNA, nuDNA, sex and temperature was used to analyze the survival data. In some comparisons we noticed significant effect of mtDNA on desiccation resistance, while all of them showed
significant effect of interaction between mitochondrial and nuclear genome. Temperature in interaction with mtDNA or mito-nuclear genotype more frequently showed significant effect on desiccation resistance compared to sex in interaction with mtDNA or mito-nuclear genotype. Our results
show adaptive significance of intra-population variation of mtDNA and importance of interactions
between mitochondrial and nuclear genome on fitness. Temperature specific mito-nuclear interaction rather than sex-specific selection on mito-nuclear genotypes maintains mtDNA variability in
this model species.
Keywords:
D. obscura; Cyt b gene; Desiccation resistance; mtDNA; VariabilityFunding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200007 (University of Belgrade, Institute for Biological Research 'Siniša Stanković') (RS-MESTD-inst-2020-200007)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200178 (University of Belgrade, Faculty of Biology) (RS-MESTD-inst-2020-200178)
In:
- Proceesings: The 1st International Electronic Conference on Entomology; 2021 Jul 1-15; Online. Basel: MDPI; 2021. p. 10522.