The content of total phenolics, flavonoids and dihydroxycinnamic acid derivatives in rhizome and vegetative parts of Corydalis ochroleuca

Abstract

Phytochemical analysis with determination of the content of secondary plant metabolites is still very popular research topic. The aim of present study was to determine the content of selected bioactive compounds in rhizome and aerial vegetative parts of the plant Corydalis ochroleuca. Plant material was collected in Derventa river canyon (Serbia). Extraction (1.5 g) was performed with 80% methanol (15 mL) as solvent for 3 h. Extracts were stored at cold and dark place until further analysis. The content of total phenolics (TPC), flavonoids (TFC), and hydroxycinnamic acid derivatives (THD) was determined via standard spectrophotometric methods and expressed as mg/g per dry weights of galic acid (GAE), quercetin (QE) and chlorogenic acid (CGAE) equivalents, respectively. It was observed that TPC, TFC and THD values were higher in vegetative part (3.45 mg/g GAE; 1.18 mg/g QE; 1.50 mg/g CGAE) compared to rhizome (2.54 mg/g GAE; 0.28 mg/g QE; 1.28 mg/g CGAE). It can be concluded that aerial vegetative parts of C. ochroleuca are better source of secondary metabolites compared to rhizome.

Ispitivanje fitohemijskog sastava biljaka i određivanje sadržaja odabranih sekundarnih metabolita u njima predstavlja i dalje veoma aktuelnu oblast istraživanja. U okviru ovog rada izvršeno je ispitivanje sadržaja odabranih sekundarnih metabolita u rizomu i nadzemnom vegetativnom delu biljke Corydalis ochroleuca. Biljni materijal je sakupljen u kanjonu reke Dervente (Srbija). Ekstrakcija (1,5 g) je izvršena pomoću 80% metanola (15 mL) u trajanju od 3h, a dobijeni ekstrakti su čuvani na hladnom i tamnom mestu. Sadržaj ukupnih polifenola (TPC), flavonoida (TFC) i derivata dihidroksicimetne kiseline (THD) je utvrđen standardnim spektrofotometrijskim metodama i izražen kao mg/g ekvivalenata galne kiseline (GAE), kvercetina (QE) i hlorogenske kiseline (CGAE) na suvu masu uzorka. Utvrđeno je da nadzemni deo biljke sadrži više bioaktivnih jedinjenja (3,45 mg/g GAE; 1,18 mg/g QE; 1,50 mg/g CGAE) u odnosu na rizom biljke (2,54 mg/g GAE; 0,28 mg/g QE; 1,28 mg/g CGAE). Na osnovu dobijenih rezultata može se reći da je nadzemni vegetativni deo biljke C. ochroleuca bolji izvor odabranih sekundarnih metabolita u odnosu na rizom.